Abstract:
OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLCC patients. Over-expression of miR-30d notably inhibited cell migration and invasion in NSCLC cell lines. miR-30d could negatively regulate Nuclear factor I B (NFIB) by directly targeting its 3'-UTR, which was confirmed by luciferase assay. NFIB also reversed miR-30d-mediated suppression on the migration and invasion in NSCLC cell lines. CONCLUSION:miR-30d suppressed cell migration and invasion by directly targeting NFIB in NSCLC, and NFIB could partially abrogated the inhibition of biological functions by miR-30d.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Wu Y,Zhang J,Hou S,Cheng Z,Yuan Mdoi
10.1007/s10529-017-2428-9subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1827-1834issue
12eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2428-9journal_volume
39pub_type
杂志文章abstract::A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9172-x
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3903-2
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1004-6
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVE:To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD. RESULTS:The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). The minimum-length substrate was agarohexaose (A6...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9757-7
更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0015-4
更新日期:2009-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0982-8
更新日期:2012-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9847-6
更新日期:2009-02-01 00:00:00
abstract::Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1501-x
更新日期:2014-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1616-0
更新日期:2014-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9821-3
更新日期:2008-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9569-1
更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9215-3
更新日期:2007-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03010-z
更新日期:2020-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1604-4
更新日期:2014-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015452.40213.bf
更新日期:2004-02-01 00:00:00
abstract::GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000013709.80691.97
更新日期:2004-02-01 00:00:00
abstract::Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6934-1
更新日期:2005-01-01 00:00:00
abstract::The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0852-4
更新日期:2012-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-009-0173-4
更新日期:2010-03-01 00:00:00
abstract:PURPOSE OF WORK:To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0564-1
更新日期:2011-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03044-3
更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021919404066
更新日期:2003-01-01 00:00:00
abstract::Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3893-0
更新日期:2005-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0380-z
更新日期:2010-12-01 00:00:00
abstract::Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025049825720
更新日期:2003-09-01 00:00:00
abstract::An optimal pooling system, called Accurate and Fast Target Screening, has been developed for high-throughput identifying the rare marker-free transformants. This system can identify targets between 10- and 100-fold more efficiently than analysis of individual samples. By calculating the efficiency for different propor...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9129-0
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03040-7
更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2450-y
更新日期:2018-01-01 00:00:00