Abstract:
:The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Chun BH,Lee YK,Chung Ndoi
10.1007/s10529-012-0982-8subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1807-10issue
10eissn
0141-5492issn
1573-6776journal_volume
34pub_type
杂志文章abstract::The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0749-7
更新日期:2012-01-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2010-09-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2003-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2020-06-01 00:00:00
abstract::Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...
journal_title:Biotechnology letters
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doi:10.1007/s10529-005-4951-3
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journal_title:Biotechnology letters
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更新日期:2016-08-01 00:00:00
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journal_title:Biotechnology letters
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doi:10.1007/s10529-006-9083-x
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journal_title:Biotechnology letters
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更新日期:2008-06-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2010-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2136-x
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli. RESULTS:Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fusion protein containe...
journal_title:Biotechnology letters
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更新日期:2018-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2081-8
更新日期:2016-07-01 00:00:00
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journal_title:Biotechnology letters
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doi:10.1007/s10529-013-1367-3
更新日期:2014-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9898-8
更新日期:2009-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0347-0
更新日期:2010-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2212-2
更新日期:2017-01-01 00:00:00
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更新日期:2015-08-01 00:00:00
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journal_title:Biotechnology letters
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doi:10.1007/s10529-013-1327-y
更新日期:2014-01-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2012-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000021962.16204.08
更新日期:2004-04-01 00:00:00
abstract::An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
abstract::Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2538-z
更新日期:2018-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1321-4
更新日期:2013-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9111-x
更新日期:2006-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-016-2160-x
更新日期:2016-10-01 00:00:00
abstract::Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2555-y
更新日期:2018-06-01 00:00:00
abstract::The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9634-4
更新日期:2008-05-01 00:00:00