Abstract:
:Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a new tool for accurate manipulation of genomic sequence to attain a therapeutic result. The advantage of CRISPR which made it an easy and flexible tool for diverse genome editing purposes is that a single protein (Cas9) complex with 2 short RNA sequences, function as a site-specific endonuclease. Recently, application of CRISPR/Cas9 system has become popular for therapeutic aims such as gene therapy. In this article, we review the fundamental mechanisms of CRISPR-Cas9 function and summarize preclinical CRISPR-mediated gene therapy reports on a wide variety of disorders.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Mollanoori H,Teimourian Sdoi
10.1007/s10529-018-2555-ysubject
Has Abstractpub_date
2018-06-01 00:00:00pages
907-914issue
6eissn
0141-5492issn
1573-6776pii
10.1007/s10529-018-2555-yjournal_volume
40pub_type
杂志文章,评审abstract:OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...
journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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更新日期:2017-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0103-5
更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9613-1
更新日期:2008-05-01 00:00:00
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doi:10.1007/s10529-011-0720-7
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-010-0295-8
更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-008-9847-6
更新日期:2009-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-012-0907-6
更新日期:2012-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-008-9855-6
更新日期:2009-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-008-9841-z
更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1023038804556
更新日期:2003-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0824-0
更新日期:2012-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-016-2190-4
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更新日期:2011-03-01 00:00:00
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更新日期:2005-08-01 00:00:00
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更新日期:2015-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-013-1405-1
更新日期:2014-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000007079.13308.2f
更新日期:2003-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2013-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2523-6
更新日期:2018-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9562-8
更新日期:2008-03-01 00:00:00