Abstract:
OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supplemented with 2 mM H2O2 for 1 h. After pretreatment with 0.5 mM H2O2, the surviving cells of L. rhamnosus hsryfm 1301 in the presence of 5 mM H2O2 for 1 h increased from 3.7 to 7.8 log CFU. Acid stress, osmotic stress, and heat stress at 46 °C also enhanced its aerotolerance, while heat stress at 50 °C reduced the tolerance of L. rhamnosus hsryfm 1301 to oxidative stress. Moreover, treatment with 0.5 mM H2O2 increased the heat stress tolerance of L. rhamnosus hsryfm 1301 by approximately 150-fold. CONCLUSIONS:Lactobacillus rhamnosus hsryfm 1301 possesses a stress-inducible defense system against oxidative stress, and the cross-adaptation to different stresses is a promising target to increase the stress tolerance of L. rhamnosus hsryfm 1301 during probiotic food and starter culture production.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhang C,Lu J,Yang D,Chen X,Huang Y,Gu Rdoi
10.1007/s10529-018-2523-6subject
Has Abstractpub_date
2018-04-01 00:00:00pages
729-735issue
4eissn
0141-5492issn
1573-6776pii
10.1007/s10529-018-2523-6journal_volume
40pub_type
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journal_title:Biotechnology letters
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pub_type: 历史文章,杂志文章,评审
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更新日期:2014-08-01 00:00:00
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更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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doi:10.1007/s10529-015-1998-7
更新日期:2016-03-01 00:00:00