Abstract:
:Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer flasks went up to 1.13 from 0.4 g l(-1), by adding 12% (w/v) resin JD-1 into the culture broth at 20 h after inoculation. In a 3 l bioreactor, pristinamycin fermentation with the addition of 12% (w/v) resin JD-1 at 20 h after inoculation reached 0.8 g l(-1), which was a 1.25-fold increase over fermentation without resin.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Jia B,Jin ZH,Lei YL,Mei LH,Li NHdoi
10.1007/s10529-006-9157-9subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1811-5issue
22eissn
0141-5492issn
1573-6776journal_volume
28pub_type
杂志文章abstract::A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0101-7
更新日期:2009-12-01 00:00:00
abstract::Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1004-6
更新日期:2012-11-01 00:00:00
abstract::Rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. In all cultures, a carbohydrate polymer fraction remained resistant to fermentation. These fractions were isolated in gram amounts and characterised. The molecular weight di...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000003975.28654.6e
更新日期:2003-11-01 00:00:00
abstract::The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9443-1
更新日期:2007-10-01 00:00:00
abstract::Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0907-6
更新日期:2012-07-01 00:00:00
abstract::Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6101-3
更新日期:2005-06-01 00:00:00
abstract:OBJECTIVES:To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species. RESULTS:The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VP...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2126-z
更新日期:2016-09-01 00:00:00
abstract::A coupled in silico thermodynamic and probabilistic metabolic control analysis methodology was verified by applying it to the glycerol biosynthetic pathway in Saccharomyces cerevisiae. The methodology allows predictions even when detailed knowledge of the enzyme kinetics is lacking. In a metabolic steady state, we fou...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1675-2
更新日期:2015-02-01 00:00:00
abstract:OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02918-w
更新日期:2020-10-01 00:00:00
abstract::Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023584805911
更新日期:2003-05-01 00:00:00
abstract::Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025003800414
更新日期:2003-08-01 00:00:00
abstract::The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...
journal_title:Biotechnology letters
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10529-017-2496-x
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1879-0
更新日期:2015-10-01 00:00:00
abstract::A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1648-5
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02658-6
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2190-4
更新日期:2016-12-01 00:00:00
abstract::In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9925-4
更新日期:2009-05-01 00:00:00
abstract::Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2723-8
更新日期:2005-11-01 00:00:00
abstract::The impact of the two adaptation-induced mutations in an improved xylose-fermenting Zymomonas mobilis strain was investigated. The chromosomal mutation at the xylose reductase gene was critical to xylose metabolism by reducing xylitol formation. Together with the plasmid-borne mutation impacting xylose isomerase activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0970-z
更新日期:2012-10-01 00:00:00
abstract::Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0960-1
更新日期:2012-09-01 00:00:00
abstract::Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9149-9
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03040-7
更新日期:2021-01-01 00:00:00
abstract::A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9172-x
更新日期:2006-12-01 00:00:00
abstract::Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0841-z
更新日期:2012-05-01 00:00:00
abstract::Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9105-8
更新日期:2006-09-01 00:00:00
abstract::A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000032966.17759.08
更新日期:2004-07-01 00:00:00
abstract::Glycolipid biosurfactants produced by bacteria and yeasts provide significant opportunities to replace chemical surfactants with sustainable biologically produced alternatives in bulk commercial products such as laundry detergents and surface cleaners. Sophorolipids are already available in sufficient yield to make th...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0956-x
更新日期:2012-09-01 00:00:00
abstract::The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1709-9
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVES:To analyze the mechanisms underlying the impact of recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)-mediated BmK IT expression on the function of baculovirus GP64 envelope fusion protein and progeny virus production. RESULTS:Viral propagation assay indicated that overexpression G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2146-8
更新日期:2016-10-01 00:00:00
abstract::1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025902.44458.f4
更新日期:2004-06-01 00:00:00