Abstract:
:A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could determine nanomolar concentrations of ATP in its fixed form via interactions with biotin-conjugated antibodies.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Nakamura M,Mie M,Funabashi H,Kobatake Edoi
10.1023/B:BILE.0000032966.17759.08keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
1061-6issue
13eissn
0141-5492issn
1573-6776pii
5379493journal_volume
26pub_type
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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更新日期:2003-07-01 00:00:00
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2013-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00