Abstract:
OBJECTIVES:To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS:An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the P malA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the P hpaII system. CONCLUSIONS:A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yue J,Fu G,Zhang D,Wen Jdoi
10.1007/s10529-017-2357-7subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1237-1244issue
8eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2357-7journal_volume
39pub_type
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