Abstract:
:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Deak PM,Lutz-Wahl S,Bothe H,Fischer Ldoi
10.1023/a:1022498314354keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
397-400issue
5eissn
0141-5492issn
1573-6776journal_volume
25pub_type
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更新日期:2004-05-01 00:00:00
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更新日期:2011-08-01 00:00:00
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更新日期:2018-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000009458.58602.d9
更新日期:2004-01-01 00:00:00
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更新日期:2020-05-01 00:00:00