Abstract:
OBJECTIVES:To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS:When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR12xHis) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps. Culturing in RB with vented caps while increasing the rotation speed from 6 rpm to 18 rpm also improved cell growth five-fold and protein productivity three-fold which is comparable to the levels observed in the orbitally shaken containers. Protein activity was found to be almost identical between the two vessel systems tested. CONCLUSIONS:Optimized cell culture conditions and a more efficient vessel type can enhance gas transfer and mixing and lead to substantial improvement of recombinant product yields from S2 cells.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Shen X,Dojcinovic D,Baldi L,Hacker DL,Luescher IF,Wurm FMdoi
10.1007/s10529-017-2440-0subject
Has Abstractpub_date
2018-01-01 00:00:00pages
85-92issue
1eissn
0141-5492issn
1573-6776pii
10.1007/s10529-017-2440-0journal_volume
40pub_type
杂志文章abstract::Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0745-y
更新日期:2012-01-01 00:00:00
abstract::5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0943-2
更新日期:2012-09-01 00:00:00
abstract::An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0568-x
更新日期:2011-07-01 00:00:00
abstract::Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0001-x
更新日期:2009-08-01 00:00:00
abstract::Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0926-3
更新日期:2012-08-01 00:00:00
abstract::In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1581-7
更新日期:2014-10-01 00:00:00
abstract::The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0663-z
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03026-5
更新日期:2021-02-01 00:00:00
abstract::A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9632-y
更新日期:2008-05-01 00:00:00
abstract::A domain of epiglucan was synthesized by beta-glucosidases. Two beta-glucosidases, an extracellular beta-glucosidase derived from Sclerotinia sclerotiorum grown on xylose, and a commercial lyophilized preparation of beta-glucosidase from Aspergillus niger, were used to synthesize gluco-oligosaccharides from cellobiose...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000023029.81600.91
更新日期:2004-04-01 00:00:00
abstract::The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at positi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9020-z
更新日期:2006-06-01 00:00:00
abstract::C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00
abstract::Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024508927361
更新日期:2003-07-01 00:00:00
abstract::A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9403-9
更新日期:2007-09-01 00:00:00
abstract::The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0382-x
更新日期:2010-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2099-y
更新日期:2016-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00
abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025016221891
更新日期:2003-08-01 00:00:00
abstract::Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
abstract:PURPOSE OF WORK:Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulator...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0413-7
更新日期:2011-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2631-3
更新日期:2019-02-01 00:00:00
abstract::We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were success...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9532-1
更新日期:2008-02-01 00:00:00
abstract::Laccase was produced by Coriolopsis rigida using barley bran as substrate in solid-state fermentation (SSF) and also by submerged fermentation (SmF). The best results were obtained in SSF with twice the amount of laccase production. Laccase could be produced from repeated batch cultures of SSF over 30 days. The laccas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9039-1
更新日期:2006-07-01 00:00:00
abstract::A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1648-5
更新日期:2015-01-01 00:00:00
abstract::Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1632-0
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02673-7
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02972-4
更新日期:2020-12-01 00:00:00
abstract::Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1004-6
更新日期:2012-11-01 00:00:00