Abstract:
:In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete the gene encoding p-nitrophenylphosphatase (PHO13) and overexpress the gene encoding transaldolase (TAL1) to evaluate the synergistic effects of these two genes on xylose fermentation in the presence of weak acid inhibitors, including formic, acetic, or levulinic acids. TAL1 over-expression or PHO13 deletion improved xylose fermentation as well as the tolerance of NAPX37 to all three weak acids. The simultaneous deletion of PHO13 and the over-expression of TAL1 had synergistic effects and improved ethanol production and reduction of xylitol accumulation in the absence and presence of weak acid inhibitors.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Li YC,Gou ZX,Liu ZS,Tang YQ,Akamatsu T,Kida Kdoi
10.1007/s10529-014-1581-7subject
Has Abstractpub_date
2014-10-01 00:00:00pages
2011-21issue
10eissn
0141-5492issn
1573-6776journal_volume
36pub_type
杂志文章abstract::Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025881.95596.23
更新日期:2004-05-01 00:00:00
abstract::Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0088-0
更新日期:2009-12-01 00:00:00
abstract::A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1648-5
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1879-0
更新日期:2015-10-01 00:00:00
abstract::Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS:A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2351-0
更新日期:2017-08-01 00:00:00
abstract::Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains OR...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9693-6
更新日期:2008-08-01 00:00:00
abstract::The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9634-4
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02734-x
更新日期:2019-11-01 00:00:00
abstract::Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9659-8
更新日期:2008-06-01 00:00:00
abstract::A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) sho...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::Environmental stresses are the major cause of crop loss worldwide. Polyamines are involved in plant stress responses. However, the precise role(s) of polyamine metabolism in these processes remain ill-defined. Transgenic approaches demonstrate that polyamines play essential roles in stress tolerance and open up the po...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9179-3
更新日期:2006-12-01 00:00:00
abstract::5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0943-2
更新日期:2012-09-01 00:00:00
abstract::Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
abstract::Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5327-4
更新日期:2006-02-01 00:00:00
abstract::Ultraviolet-B (UV-B) radiation has significantly negative effect on cell survival rate (P < 0.01) and positive effect on astaxanthin accumulation (P < 0.01) of Haematococcus pluvialis. H. pluvialis accumulated 3.2 mg/g of astaxanthin when being exposed to 5 W/m(2) of UV-B for 60 min prior to 72 h of high light treatme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0371-0
更新日期:2010-12-01 00:00:00
abstract::The volumetric productivity of the beer fermentation process can be increased by using a higher pitching rate (i.e. higher inoculum size). However, the decreased yeast net growth observed in these high cell density brewery fermentations can adversely affect the physiological stability throughout subsequent yeast gener...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0067-5
更新日期:2009-11-01 00:00:00
abstract::We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0544-5
更新日期:2011-06-01 00:00:00
abstract::Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0684-6
更新日期:2005-03-01 00:00:00
abstract::Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0857-z
更新日期:2012-05-01 00:00:00
abstract::Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0504-5
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02850-z
更新日期:2020-06-01 00:00:00
abstract::Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4951-3
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02658-6
更新日期:2019-05-01 00:00:00
abstract::Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9651-3
更新日期:2008-06-01 00:00:00
abstract::Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-2010-2
更新日期:2016-04-01 00:00:00
abstract::The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2376-4
更新日期:2017-09-01 00:00:00
abstract::In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1730-z
更新日期:2015-03-01 00:00:00
abstract::Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00
abstract::The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022498314354
更新日期:2003-03-01 00:00:00