Abstract:
:Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60 % without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Hao G,Chen H,Du K,Huang X,Song Y,Gu Z,Wang L,Zhang H,Chen W,Chen YQdoi
10.1007/s10529-014-1546-xsubject
Has Abstractpub_date
2014-09-01 00:00:00pages
1827-34issue
9eissn
0141-5492issn
1573-6776journal_volume
36pub_type
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