Abstract:
OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of adhesion molecules, ICAM-1, VCAM-1 and E-selectin (mRNA and protein), augmented the adhesion of THP-1 cells to HUVECs, activated NF-κB binding, increased the release of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and induced oxidative damage (reduced glutathione declined, while 8-OHdG and TBARS increased). 5 µM DNC significantly inhibited these radiation-induced changes, activated the Nrf-2 pathway, and effectively suppressed THP-1 adhesion to HUVECs, implicating p38 MAPK signaling. CONCLUSION:DNC treatment is a potential preventive method against inflammation and vascular damage from ionizing radiation.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh Mdoi
10.1007/s10529-016-2189-xsubject
Has Abstractpub_date
2016-12-01 00:00:00pages
2081-2088issue
12eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2189-xjournal_volume
38pub_type
杂志文章abstract::Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5327-4
更新日期:2006-02-01 00:00:00
abstract::Ma-pyrG was cloned from Monascus aurantiacus AS3.4384 using degenerate PCR with primers designed with an algorithm called CODEHOP, and its complete sequence was obtained by a PCR-based strategy for screening a Monascus fosmid library. Ma-pyrG encodes orotidine-5'-phosphate decarboxylase (OMPdecase), a 283-aminoacid pr...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0336-3
更新日期:2010-11-01 00:00:00
abstract::The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0797-z
更新日期:2012-03-01 00:00:00
abstract::An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from D-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1400-6
更新日期:2014-03-01 00:00:00
abstract::Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025003800414
更新日期:2003-08-01 00:00:00
abstract::Rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. In all cultures, a carbohydrate polymer fraction remained resistant to fermentation. These fractions were isolated in gram amounts and characterised. The molecular weight di...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000003975.28654.6e
更新日期:2003-11-01 00:00:00
abstract::An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0568-x
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress. RESULTS:A Δmpx mutant exhibited signific...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2099-y
更新日期:2016-07-01 00:00:00
abstract::A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1293-4
更新日期:2013-11-01 00:00:00
abstract::An endo-beta-(1-->3),(1-->4)-glucanase gene (bglBC1) from Bacillus circulans ATCC21367 was modified by substituting its native promoter with a strong promoter, BJ27X, to increase expression of the gene when cloned into B. subtilis RM125 and B. megaterium ATCC14945. A 771-bp endo-beta-(1-->3),(1-->4)-glucanase open rea...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-4581-1
更新日期:2004-11-01 00:00:00
abstract:OBJECTS:To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients. RESULTS:GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2077-4
更新日期:2016-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::Growth and sporulation of Verticillium lecanii on inert and organic carriers (sugar-cane bagasse, corncob, rice straw, polyurethane foam and activated carbon) in a solid-state fermentation process was studied. Sugar-cane bagasse and polyurethane foam produced 10(10) spores g(-1) dry carrier whereas corncob, rice straw...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0496-1
更新日期:2011-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03021-w
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVE:To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production. RESULTS:Monoamine oxidase, originating from Aspergillus niger and subj...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2450-y
更新日期:2018-01-01 00:00:00
abstract::There has been an increasing interest in applying biotechnology in formulating and characterising new and innovative drug delivery methods, e.g., drug-loaded biodegradable microneedles within the area of transdermal delivery technology. Recently, microneedles have been proposed for use in pain management, e.g., post-o...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1217-3
更新日期:2013-09-01 00:00:00
abstract::A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9821-3
更新日期:2008-12-01 00:00:00
abstract::By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0456-9
更新日期:2011-03-01 00:00:00
abstract::Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0684-6
更新日期:2005-03-01 00:00:00
abstract::GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000013709.80691.97
更新日期:2004-02-01 00:00:00
abstract::Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0745-y
更新日期:2012-01-01 00:00:00
abstract::Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0026-3
更新日期:2006-05-01 00:00:00
abstract::Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000009458.58602.d9
更新日期:2004-01-01 00:00:00
abstract::This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-004-4579-8
更新日期:2004-11-01 00:00:00
abstract::Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2516-5
更新日期:2018-04-01 00:00:00
abstract::To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1489-2
更新日期:2014-06-01 00:00:00
abstract::The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1140-7
更新日期:2013-05-01 00:00:00
abstract::A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000012891.13904.62
更新日期:2004-01-01 00:00:00
abstract::A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1648-5
更新日期:2015-01-01 00:00:00