Increase in D-tagatose production rate by site-directed mutagenesis of L-arabinose isomerase from Geobacillus thermodenitrificans.

abstract：:The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...

journal_title：Biotechnology letters

authors： Li W,Chen Y,Sheng Y,Xie Z,Tang Y

更新日期：2018-01-01 00:00:00

abstract：:Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

journal_title：Biotechnology letters

authors： Adamitsch BF,Karner F,Hampel WA

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：:This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...

journal_title：Biotechnology letters

authors： Hildén L,Johansson G

更新日期：2004-11-01 00:00:00

abstract：:The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...

journal_title：Biotechnology letters

authors： Li Y,Liu L,Tian P

更新日期：2014-11-01 00:00:00

abstract：:The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...

journal_title：Biotechnology letters

authors： Kim SI,Park SH,Kim JW,Leem SH,Shin DJ,Kim SH,Lee DH,Kahng HY

更新日期：2007-10-01 00:00:00

abstract：:Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...

journal_title：Biotechnology letters

authors： Berne C,Allainmat B,Garcia D

更新日期：2005-04-01 00:00:00

abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...

journal_title：Biotechnology letters

authors： Turner KM,Pasut G,Veronese FM,Boyce A,Walsh G

更新日期：2011-03-01 00:00:00

abstract：:Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...

journal_title：Biotechnology letters

authors： Díaz-Barrera A,Silva P,Avalos R,Acevedo F

更新日期：2009-06-01 00:00:00

abstract：:Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Mor...

journal_title：Biotechnology letters

authors： Tang M,Zhang RQ,Chen H,Zhang HH,Tian ZQ

更新日期：2008-10-01 00:00:00

abstract：:The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...

journal_title：Biotechnology letters

authors： Camperi SA,Iannucci NB,Albanesi GJ,Oggero Eberhardt M,Etcheverrigaray M,Messeguer A,Albericio F,Cascone O

更新日期：2003-09-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00

abstract：OBJECTIVES:This short commentary examines the factors that led to Food and Drug Administration's approval of the first plant-derived biologic. RESULTS:In 2012, the first plant-derived protein pharmaceutical (biologic) was approved for commercial use in humans. The product, a recombinant form of human β-glucocerebrosid...

journal_title：Biotechnology letters

authors： Mor TS

更新日期：2015-11-01 00:00:00

abstract：:A prospective clinical parametric study comprising women afflicted by breast cancer and otherwise healthy participants was undertaken. The mean plasmatic concentration of putative leucine amino peptidase and nucleoside diphosphate phosphotransferase enzymatic complex in breast cancer cases was significantly elevated [...

journal_title：Biotechnology letters

authors： Pulido-Cejudo G,Miranda H,El Abdaimi K,Wang C,Kar B,Medina Acevedo J,Cárdenas JM,Sarti Gutiérrez E,Pérez Palacios G

更新日期：2004-09-01 00:00:00

abstract：:Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The proces...

journal_title：Biotechnology letters

authors： Ferreira S,Sousa F,Queiroz JA,Domingues FC

更新日期：2005-08-01 00:00:00

abstract：OBJECTIVES:The objective of this study was to discover unknown differentially expressed genes (DEGs) associated with bronchopulmonary dysplasia (BPD), analyze their functions and enriched signaling pathways, and identify hub genes correlating with BPD incidence and evolvement. RESULTS:Of 1289 DEGs identified, 568 were...

journal_title：Biotechnology letters

authors： Yan W,Jiang M,Zheng J

更新日期：2020-12-01 00:00:00

abstract：:The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...

journal_title：Biotechnology letters

authors： van der Valk AM,Howbrook DN,O'Shaughnessy MC,Sarker DK,Baker SC,Louwrier A,Lloyd AW

更新日期：2003-08-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：:The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD ...

journal_title：Biotechnology letters

authors： Iannaccone M,Palumbo D,Ventimiglia I,Patocchi A,Spigno P,Capparelli R

更新日期：2007-02-01 00:00:00

abstract：OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...

journal_title：Biotechnology letters

authors： Shariati L,Modaress M,Khanahmad H,Hejazi Z,Tabatabaiefar MA,Salehi M,Modarressi MH

更新日期：2016-08-01 00:00:00

abstract：:Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...

journal_title：Biotechnology letters

authors： Li Y,Zhang T,Shen ZW,Xu Y,Li JY

更新日期：2013-01-01 00:00:00

abstract：:Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA(3), was integrated into L. ...

journal_title：Biotechnology letters

authors： Wang L,Li X,Chen S,Liu G

更新日期：2009-02-01 00:00:00

abstract：:Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of extracellular glyc...

journal_title：Biotechnology letters

authors： Apte-Deshpnade A,Mandal G,Soorapaneni S,Prasad B,Kumar J,Padmanabhan S

更新日期：2009-06-01 00:00:00

abstract：:Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...

journal_title：Biotechnology letters

authors： Mitsukura K,Imoto T,Nagaoka H,Yoshida T,Nagasawa T

更新日期：2005-09-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00

abstract：:A hybrid baculovirus, a hybrid of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (IL-21) in silkworms. A recombinant hybrid baculovirus containing the full length of the cDNA of bovine interleukin-21...

journal_title：Biotechnology letters

authors： Muneta Y,Nagaya H,Minagawa Y,Enomoto C,Matsumoto S,Mori Y

更新日期：2004-09-01 00:00:00

abstract：:Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...

journal_title：Biotechnology letters

authors： Li IT,Pham E,Truong K

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVES:Regenerative medicine approaches using reprogrammed or transdifferentiated cells require efficient single cell expression profiling to analyze culture homogeneity for quality control and recipients' safety. RESULTS:While antigen-antibody based systems have been developed for several proteins, probing at the...

journal_title：Biotechnology letters

authors： Kraus P,Kocsis V,Williams C,Youngs B,Lufkin T

更新日期：2015-08-01 00:00:00

abstract：:Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...

journal_title：Biotechnology letters

authors： Tang X,Zheng J,Yan Q,Li Z,Li Y

更新日期：2013-06-01 00:00:00

abstract：OBJECTIVES:To improve the thermostability and catalytic property of a mesophilic 1,3-1,4-β-glucanase by combinational mutagenesis and to test its effect in congress mashing. RESULTS:A mutant β-glucanase (rE-BglTO) constructed by combinational mutagenesis showed a 25 °C increase in optimal temperature (to 70 °C) a 19.5...

journal_title：Biotechnology letters

authors： Niu C,Zhu L,Hill A,Alex Speers R,Li Q

更新日期：2017-01-01 00:00:00