Abstract:
OBJECTIVES:Regenerative medicine approaches using reprogrammed or transdifferentiated cells require efficient single cell expression profiling to analyze culture homogeneity for quality control and recipients' safety. RESULTS:While antigen-antibody based systems have been developed for several proteins, probing at the mRNA level allows for more flexibility, faster adaption to the ever increasing new data from next generation sequencing and increased specificity, especially for genes of conserved gene families. CONCLUSIONS:We developed a time and cost effective expression profiling assay for monolayer cell culture in 96-well plates based on RNA in situ hybridization, termed PISH, at single cell resolution.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Kraus P,Kocsis V,Williams C,Youngs B,Lufkin Tdoi
10.1007/s10529-015-1833-1subject
Has Abstractpub_date
2015-08-01 00:00:00pages
1573-7issue
8eissn
0141-5492issn
1573-6776journal_volume
37pub_type
杂志文章abstract::The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0720-7
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0741-2
更新日期:2012-01-01 00:00:00
abstract::The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0650-4
更新日期:2011-10-01 00:00:00
abstract::Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023038804556
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVES:To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. RESULTS:A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metageno...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1857-6
更新日期:2015-09-01 00:00:00
abstract::Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6032-4
更新日期:2004-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02918-w
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1896-z
更新日期:2015-11-01 00:00:00
abstract:PURPOSE OF WORK:To apply a fluorescent dye as an alternative technique to evaluate the survival of potentially probiotic lactobacilli to bile acids (BA) as first step in the design of probiotic functional foods. The use of lactic acid bacteria (LAB) in the functional food design depends on their ability to survive in t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0564-1
更新日期:2011-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024562900548
更新日期:2003-07-01 00:00:00
abstract::An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9140-5
更新日期:2006-10-01 00:00:00
abstract::Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9938-z
更新日期:2009-06-01 00:00:00
abstract::Two genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatog...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0762-x
更新日期:2012-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02696-0
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00
abstract::Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2555-y
更新日期:2018-06-01 00:00:00
abstract::Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5327-4
更新日期:2006-02-01 00:00:00
abstract::GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000013709.80691.97
更新日期:2004-02-01 00:00:00
abstract::Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0985-5
更新日期:2012-10-01 00:00:00
abstract::Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1146-1
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1924-z
更新日期:2015-12-01 00:00:00
abstract::Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9122-7
更新日期:2006-10-01 00:00:00
abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0160-9
更新日期:2010-03-01 00:00:00
abstract::Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2516-5
更新日期:2018-04-01 00:00:00
abstract::Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open readin...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0269-x
更新日期:2010-08-01 00:00:00
abstract::Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022890119847
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03021-w
更新日期:2021-01-01 00:00:00
abstract::The thermostablility and enzymatic activity of 1,3-1,4-β-glucanase (BglA) from Bacillus amyloliquefaciens was improved by modifying five (out of 12) ε-amino groups in lysine residues with nitrous acid. The optimal modification condition for BglA was determined as 30 mM nitrous acid at, 40 °C for 30 min. The optimally-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1616-0
更新日期:2014-12-01 00:00:00