Abstract:
OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp and the open reading frame encoded 418 amino acids. The efeb gene was expressed in Escherichia coli BL21 and a recombinant BaDyP of 50 kDa was achieved. The BaDyP exhibited activity in oxidizing GGE and decolorizing azo and triphenylmethane dyes. At pH 4.5 and 30 °C the BaDyP not only completely degraded GGE by the cleavage of β-O-4 ether bond and Cα-Cβ bond, and Cα oxidation without any oxidative mediator, but also decolorized four synthetic dyes, including congo red, bromine cresol green, eriochrome black T and crystal violet. This was achieved with decolorization rates of 65.7%, 70.62%, 80.06% and 62.09%, respectively, after 72 h of incubation. CONCLUSIONS:BaDyP was identified as a bacteria peroxidase with great potential for the degradation of lignin and bioremediation of dye-contamination.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang Sdoi
10.1007/s10529-019-02696-0subject
Has Abstractpub_date
2019-09-01 00:00:00pages
1015-1021issue
8-9eissn
0141-5492issn
1573-6776pii
10.1007/s10529-019-02696-0journal_volume
41pub_type
杂志文章abstract::Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with gr...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1670-7
更新日期:2015-01-01 00:00:00
abstract::The rhizosphere is the soil-plant root interphase and in practice consists of the soil adhering to the root besides the loose soil surrounding it. Plant growth-promoting rhizobacteria (PGPR) are potential agents for the biological control of plant pathogens. A biocontrol strain should be able to protect the host plant...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0347-0
更新日期:2010-11-01 00:00:00
abstract::Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1596-0
更新日期:2014-11-01 00:00:00
abstract::Fifty-two fungal isolates obtained from seawater were screened for anti-nematode activity. One isolate, an Acremonium sp., exhibited the highest activity against the pinewood nematode, Bursaphelenchus xylophilus. When it was grown at pH 7.0 and 24-26 °C, nematicidal metabolites were produced. These were heat-stable, w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0967-7
更新日期:2012-10-01 00:00:00
abstract::A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0295-8
更新日期:2010-09-01 00:00:00
abstract::The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1291-6
更新日期:2013-11-01 00:00:00
abstract::Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
abstract::L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned fro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9885-0
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2469-0
更新日期:2018-02-01 00:00:00
abstract::A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9632-y
更新日期:2008-05-01 00:00:00
abstract::Four Cr(VI)-reducing bacterial strains (Ochrobactrum intermedium, CrT-2, CrT-3 and CrT-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (Helianthus annuus var SF-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg CrCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7188-2
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
abstract::The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1590-6
更新日期:2014-11-01 00:00:00
abstract::A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9215-3
更新日期:2007-01-01 00:00:00
abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025016221891
更新日期:2003-08-01 00:00:00
abstract::Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3893-0
更新日期:2005-12-01 00:00:00
abstract::A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0404-8
更新日期:2011-01-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract::The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0210-3
更新日期:2010-05-01 00:00:00
abstract::Centaurea calcitrapa suspension cultures were grown either in Erlenmeyer flasks or in a mechanically stirred bioreactor. Its rheological behaviour, when fitted to the Oswald-de Waele model (power law), showed pseudoplastic characteristics in both cases. The flow behaviour index (n) decreased over the course of a growt...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6181-0
更新日期:2006-03-01 00:00:00
abstract::Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2555-y
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166(T). RESULTS:Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1971-5
更新日期:2016-02-01 00:00:00
abstract::Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9193-5
更新日期:2006-12-01 00:00:00
abstract::The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1691-2
更新日期:2015-02-01 00:00:00
abstract::Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition u...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0443-1
更新日期:2011-02-01 00:00:00
abstract::A strain of Pseudomonas stutzeri ZWLR2-1 was isolated from soil contaminated with chloronitrobenzenes and identified by 16S rDNA sequencing. This bacterium released chloride and nitrite into the medium when grown on 0.5 mM 2-chloronitrobenzene. PCR amplification and DNA sequencing revealed a DNA fragment encoding a po...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-8293-3
更新日期:2005-02-01 00:00:00
abstract::This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-004-4579-8
更新日期:2004-11-01 00:00:00
abstract::Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025072020791
更新日期:2003-09-01 00:00:00
abstract::O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0178-z
更新日期:2010-04-01 00:00:00