Overexpression of a soybean O-acetylserine (thiol) lyase-encoding gene GmOASTL4 in tobacco increases cysteine levels and enhances tolerance to cadmium stress.

abstract：:A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...

journal_title：Biotechnology letters

authors： Woo JH,Kwon TH,Kim JT,Kim CG,Lee EY

更新日期：2013-04-01 00:00:00

abstract：:The stability (half-life, t(½)) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t(½) approximately 8.5 months at pH ≤ 6) versus alkaline conditions (t(½) approximately 15 months at pH 8-12). Acidic conditions induce the dissociation of haem from CAT, as reve...

journal_title：Biotechnology letters

authors： Vatsyayan P,Goswami P

更新日期：2011-02-01 00:00:00

abstract：:Efforts to elucidate the relationships between microorganisms and metal corrosion were mainly directed to understanding the formation of biofilm structures grown on corroded surfaces. The emergence of high throughput DNA sequencing techniques has helped in the description of microbial species involved directly and ind...

journal_title：Biotechnology letters

authors： Procópio L

更新日期：2020-03-01 00:00:00

abstract：:Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content ...

journal_title：Biotechnology letters

authors： Lin Y,Fang G,Peng K

更新日期：2007-02-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were ...

journal_title：Biotechnology letters

authors： Birla RK,Dhawan V,Dow DE,Huang YC,Brown DL

更新日期：2009-02-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...

journal_title：Biotechnology letters

authors： Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu F

更新日期：2012-10-01 00:00:00

abstract：:Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...

journal_title：Biotechnology letters

authors： Yao B,Zhao Y,Zhang H,Zhang M,Liu M,Liu H,Li J

更新日期：2012-05-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：OBJECTIVES:To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166(T). RESULTS:Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 ...

journal_title：Biotechnology letters

authors： Wang Y,Yu W,Han F

更新日期：2016-02-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：:A recombinant plasmid, pGMF, containing a gamma-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells...

journal_title：Biotechnology letters

authors： Fan X,He X,Guo X,Qu N,Wang C,Zhang B

更新日期：2004-03-01 00:00:00

abstract：:The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...

journal_title：Biotechnology letters

authors： Park CS,Yeom SJ,Kim HJ,Lee SH,Lee JK,Kim SW,Oh DK

更新日期：2007-09-01 00:00:00

abstract：:We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known...

journal_title：Biotechnology letters

authors： Kim M,Yun CH,Park SK,Seo JH,Ahn T

更新日期：2007-11-01 00:00:00

abstract：:Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...

journal_title：Biotechnology letters

authors： Etschmann MM,Sell D,Schrader J

更新日期：2003-04-01 00:00:00

abstract：:An unstructured model based on mass balance equations for a recombinant methylotrophic yeast Pichia pastoris MutS (Methanol Utilization Slow) strain expressing the mini-proinsulin (MPI), was successfully established in quasi-steady state fed-batch fermentations with varying total quantity of biomass in a 7 l fermenter...

journal_title：Biotechnology letters

authors： País JM,Varas L,Valdés J,Cabello C,Rodríguez L,Mansur M

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

journal_title：Biotechnology letters

authors： Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

更新日期：2021-01-01 00:00:00

abstract：OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...

journal_title：Biotechnology letters

authors： Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi T

更新日期：2021-01-26 00:00:00

abstract：OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...

journal_title：Biotechnology letters

authors： Stepanov A,Belyy A,Kasheverov I,Rybinets A,Dronina M,Dyachenko I,Murashev A,Knorre V,Sakharov D,Ponomarenko N,Tsetlin V,Tonevitsky A,Deyev S,Belogurov A Jr,Gabibov A

更新日期：2016-07-01 00:00:00

abstract：:Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains OR...

journal_title：Biotechnology letters

authors： Senthilkumar M,Madhaiyan M,Sundaram SP,Sangeetha H,Kannaiyan S

更新日期：2008-08-01 00:00:00

abstract：:The gene encoding Schwanniomyces occidentalis alpha-amylase (AMY) was introduced into the chromosomal delta sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an delta-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AM...

journal_title：Biotechnology letters

authors： Kang NY,Park JN,Chin JE,Lee HB,Im SY,Bai S

更新日期：2003-11-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：:Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with ...

journal_title：Biotechnology letters

authors： Fu CX,Zhao DX,Huang Y,Ma FS

更新日期：2005-01-01 00:00:00

abstract：:Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with gr...

journal_title：Biotechnology letters

authors： He YC,Tao ZC,Zhang DP,Yang ZX,Gao S,Ma CL

更新日期：2015-01-01 00:00:00

abstract：:Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...

journal_title：Biotechnology letters

authors： Han DW,Sub Lee M,Park BJ,Kim JK,Park JC

更新日期：2005-01-01 00:00:00

abstract：OBJECTIVE:To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS:Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulat...

journal_title：Biotechnology letters

authors： Diao X,Sun S

更新日期：2017-09-01 00:00:00

abstract：:The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...

journal_title：Biotechnology letters

authors： Shatila F,Yaşa İ,Yalçın HT

更新日期：2020-10-01 00:00:00