Abstract:
:L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10 degrees C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Baik SH,Yoshioka Hdoi
10.1007/s10529-008-9885-0subject
Has Abstractpub_date
2009-03-01 00:00:00pages
443-8issue
3eissn
0141-5492issn
1573-6776journal_volume
31pub_type
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更新日期:2013-12-01 00:00:00
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2017-09-01 00:00:00
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更新日期:2015-07-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2005-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-8293-3
更新日期:2005-02-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2005-11-01 00:00:00