Abstract:
OBJECTIVES:To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. RESULTS:The elongation factor P (EF-P) promoter from Bifidobacterium longum CECT 4551 produced the highest emission of fluorescence signal and was therefore able to produce the highest gene expression of the promoters studied. The promoters from B. longum CECT 4551 showed different fluorescence signal intensities which, in descending order, were: EF-P, initiation factor IF-2, elongation factor G, elongation factor Tu, elongation factor Nus A, elongation factor Ts and 30S ribosomal protein S12. CONCLUSIONS:The consistency of the methods employed (fluorescence imaging system, fluorescence microscopy, fluorimetry and flow cytometry) showed that the construction pNZ:Prom.GFPana contained the anaerobic fluorescent protein evoglow-Pp1 could be exploited as a tool for analysing the gene expression in bifidobacteria strains.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Montenegro-Rodríguez C,Peirotén A,Sanchez-Jimenez A,Arqués JL,Landete JMdoi
10.1007/s10529-015-1802-8subject
Has Abstractpub_date
2015-07-01 00:00:00pages
1405-13issue
7eissn
0141-5492issn
1573-6776journal_volume
37pub_type
杂志文章abstract::To understand how molecular damage under harsh environmental conditions can be controlled, we investigated the properties of ATPase activity of the chaperonin molecular machinery from the hyperthermophilic archaeon Pyrococcus furiosus (PfCPN). PfCPN ATPase activity depended on K(+) and Mg(2+) and its optimal pH was 7....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0070-x
更新日期:2009-11-01 00:00:00
abstract:OBJECTIVES:To identify a new member of serine proteases from Deinagkistrodon acutus via phage display technique and appraise its biocatalytic activities. RESULTS:A novel thrombin-like enzyme gene was cloned by screening the phage display library of D. acutus venom gland. The gene has a 783 bp ORF encoding 260 amino ac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2441-z
更新日期:2018-01-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract::Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
更新日期:2013-06-01 00:00:00
abstract::Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0284-y
更新日期:2010-09-01 00:00:00
abstract::Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-005-1521-7
更新日期:2005-03-01 00:00:00
abstract::Degradation of lignocellulose with pressurised hot water is an efficient method of bioethanol production. However, the resultant solution inhibits ethanol fermentation by Saccharomyces cerevisiae. Here, we first report that glycolaldehyde, which is formed when lignocellulose is treated with pressurised hot water, inhi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0437-z
更新日期:2011-02-01 00:00:00
abstract::A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000032966.17759.08
更新日期:2004-07-01 00:00:00
abstract::The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed und...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9280-7
更新日期:2007-04-01 00:00:00
abstract:OBJECTIVES:L-isoleucine dioxygenase (IDO) specifically transforms L-isoleucine (Ile) to 4-hydroxyisoleucine (4-HIL), and 4-HIL is a promising drug for diabetes. To enhance the activity and catalytic efficiency of IDO, we used directed evolution and site-specific mutagenesis. RESULTS:The IDO gene (ido) derived from Bac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2566-8
更新日期:2018-08-01 00:00:00
abstract::The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI wa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9083-x
更新日期:2006-08-01 00:00:00
abstract::As more and more cell and gene therapies are being developed and with the increasing number of regulatory approvals being obtained, there is an emerging and pressing need for industrial translation. Process efficiency, associated cost drivers and regulatory requirements are issues that need to be addressed before indu...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-019-02732-z
更新日期:2019-11-01 00:00:00
abstract::The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2376-4
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
abstract::Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9949-9
更新日期:2009-06-01 00:00:00
abstract::The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9167-7
更新日期:2006-11-01 00:00:00
abstract::Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0048-8
更新日期:2009-10-01 00:00:00
abstract::Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptak...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1263-x
更新日期:2013-11-01 00:00:00
abstract::Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0533-8
更新日期:2011-05-01 00:00:00
abstract::Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0606-8
更新日期:2011-08-01 00:00:00
abstract::Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0926-3
更新日期:2012-08-01 00:00:00
abstract::Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6032-4
更新日期:2004-12-01 00:00:00
abstract::Nitrite at 2 mM did not affect the growth of Botryococcus braunii and served as the sole nitrogen source giving a minimum biomass doubling time of 4.2 d, which was equal to that of the culture using 4 mM nitrate as nitrogen source. With nitrite at 4 mM, after a lag phase of about 10 d, the alga grew quickly, reaching ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000013722.45527.18
更新日期:2004-02-01 00:00:00
abstract::Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0592-x
更新日期:2011-08-01 00:00:00
abstract::A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0184-1
更新日期:2010-04-01 00:00:00
abstract:PURPOSE OF WORK:To increase the bioproduction of L-phenylacetylcarbinol (PAC), a precursor molecule in the synthesis of the decongestants ephedrine and pseudoephedrine and which suffers from substrate, product, and by-product inhibition, by ensuring that the delivery of the substrate, benzaldehyde, is maintained within...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0408-4
更新日期:2011-01-01 00:00:00
abstract::Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00
abstract::Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract::3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0210-3
更新日期:2010-05-01 00:00:00
abstract::Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0635-3
更新日期:2011-09-01 00:00:00