Industrial production of fructooligosaccharides by immobilized cells of Aureobasidium pullulans in a packed bed reactor.

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00

abstract：:The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...

journal_title：Biotechnology letters

authors： Lee JH,Kim YT

更新日期：2006-08-01 00:00:00

abstract：OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

journal_title：Biotechnology letters

authors： Wiegmann V,Martinez CB,Baganz F

更新日期：2018-07-01 00:00:00

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：:Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 ...

journal_title：Biotechnology letters

authors： Müller C,Wagner K,Frankenfeld K,Franzreb M

更新日期：2011-05-01 00:00:00

abstract：OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

journal_title：Biotechnology letters

authors： Li K,Varden L,Henderson A,Lufkin T,Kraus P

更新日期：2021-01-01 00:00:00

abstract：:The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

journal_title：Biotechnology letters

authors： Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

更新日期：2021-01-02 00:00:00

abstract：:Enzymatic polymerization can offer metal-free routes to polymer materials that could be used in biomedical applications. To take advantage of the unique properties of ionic liquids (ILs) for enzyme stability, monocationic ionic liquid (MIL) and dicationic ionic liquid (DIL) were used to promote the ring-opening polyme...

journal_title：Biotechnology letters

authors： Wu C,Zhang Z,He F,Zhuo R

更新日期：2013-06-01 00:00:00

abstract：OBJECTIVES:L-isoleucine dioxygenase (IDO) specifically transforms L-isoleucine (Ile) to 4-hydroxyisoleucine (4-HIL), and 4-HIL is a promising drug for diabetes. To enhance the activity and catalytic efficiency of IDO, we used directed evolution and site-specific mutagenesis. RESULTS:The IDO gene (ido) derived from Bac...

journal_title：Biotechnology letters

authors： Huang S,Shi F

更新日期：2018-08-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：:The use of H(2), He and O(2) during batch fermentation of Saccharomyces cerevisiae BRAS291 increased the final intracellular glycogen contents of the cells from 2-fold to 10-fold compared with a gas-free condition, and this depended on the gas applied. Differently, the intracellular trehalose contents increased from 2...

journal_title：Biotechnology letters

authors： Pham TH,Mauvais G,Vergoignan C,De Coninck J,Cachon R,Feron G

更新日期：2008-02-01 00:00:00

abstract：:Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...

journal_title：Biotechnology letters

authors： Liu Y,Zhou X,Song Z,Zhang Y

更新日期：2014-05-01 00:00:00

abstract：:Chlorella vulgaris is a form of microalgae commonly employed as a biological source of oil for biodiesel production. Major algal cultivation strategies are focused on stimulating growth rate and lipid content. In the present study, the algal growth media was supplemented with iron (III) chloride (FeCl3), as a stimulat...

journal_title：Biotechnology letters

authors： Khamoushi A,Tafakori V,Zahed MA,Gayglou SE,Angaji SA

更新日期：2020-12-01 00:00:00

abstract：:Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cell...

journal_title：Biotechnology letters

authors： Fraccaroli M,Nicoletti S,Maltese F,Choi YH,Guzzo F,Levi M,Verpoorte R

更新日期：2008-11-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：:Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...

journal_title：Biotechnology letters

authors： Fu Y,Zheng S,Huang R,An N,Zheng Y,Zhang Z,Liang A

更新日期：2012-01-01 00:00:00

abstract：:Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin alpha and L: -beta-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is we...

journal_title：Biotechnology letters

authors： Abrunhosa L,Venâncio A

更新日期：2007-12-01 00:00:00

abstract：:Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...

journal_title：Biotechnology letters

authors： Mukundan LM,Nirmal R,Thomas LV,Sajeev US,Nair PD

更新日期：2011-10-01 00:00:00

abstract：OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...

journal_title：Biotechnology letters

authors： Zhang Y,Yang L,Ling C,Heng W

更新日期：2018-04-01 00:00:00

abstract：:The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...

journal_title：Biotechnology letters

authors： Yang Y,Wang J,Yuan T,Bu D,Yang J,Sun P

更新日期：2013-11-01 00:00:00

abstract：OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...

journal_title：Biotechnology letters

authors： Nakazato G,Gonçalves MC,da Silva das Neves M,Kobayashi RKT,Brocchi M,Durán N

更新日期：2019-12-01 00:00:00

abstract：:The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF(4)]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H(2)O(2) were investigated. The ionic liquid stabilized the ground state of ...

journal_title：Biotechnology letters

authors： Hong ES,Kwon OY,Ryu K

更新日期：2008-03-01 00:00:00

abstract：:δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...

journal_title：Biotechnology letters

authors： Liu L,Liu C,Zou S,Yang H,Hong J,Ma Y,Zhang M

更新日期：2013-08-01 00:00:00

abstract：OBJECTIVES:To prepare recombinant tPep-(vascular endothelial growth factor) VEGF-B and assess its biological activity. RESULTS:This new VEGF fusion protein was constructed using a targeting peptide and prepared using E.coli. The tPep-VEGF-B was refolded from inclusion bodies and purified using affinity chromatography....

journal_title：Biotechnology letters

authors： Zhang Y,Tong Y,Gao M,Luo C,Song X,Gao X,Yao W

更新日期：2016-07-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：OBJECTIVES:To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. RESULTS:Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical...

journal_title：Biotechnology letters

authors： Yang X,Yuan Q,Zheng Y,Ma H,Chen T,Zhao X

更新日期：2016-08-01 00:00:00

abstract：OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...

journal_title：Biotechnology letters

authors： Hu L,Chen X,Chen M,Fang J,Nie J,Dai H

更新日期：2021-01-01 00:00:00

abstract：OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we f...

journal_title：Biotechnology letters

authors： Huang X,Zheng M,Yi Y,Patel A,Song Z,Li Y

更新日期：2020-11-01 00:00:00

abstract：:The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitat...

journal_title：Biotechnology letters

authors： Grosjean F,Bertschinger M,Hacker DL,Wurm FM

更新日期：2006-11-01 00:00:00