Abstract:
OBJECTIVES:To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. RESULTS:Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical acetone yield from 1 to 1.5 mol acetone/mol glucose. By inserting the fxpk gene encoding phosphoketolase from Bifidobacterium adolescentis into the genome, we constructed a NOG pathway in E.coli. The resulting strain produced 47 mM acetone from glucose under aerobic conditions in shake-flasks. The yield of acetone was improved from 0.38 to 0.47 mol acetone/mol glucose which is a significant over the parent strain. CONCLUSIONS:Guided by computational analysis of metabolic networks, we introduced a NOG pathway into E. coli and increased the yield of acetone, which demonstrates the importance of modeling analysis for the novel metabolic engineering strategies.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yang X,Yuan Q,Zheng Y,Ma H,Chen T,Zhao Xdoi
10.1007/s10529-016-2115-2subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1359-65issue
8eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2115-2journal_volume
38pub_type
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