Effective biotic elicitation of Ruta graveolens L. shoot cultures by lysates from Pectobacterium atrosepticum and Bacillus sp.

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:We report a novel and convenient method for the determination of glycoproteins, especially antibodies, using galactose oxidase (GAO) on the basis of the contents of galactosyl and N-acetylgalactosaminyl residues in carbohydrate chains of glycoproteins. GAO converts galactose residues to their corresponding aldehyde an...

journal_title：Biotechnology letters

authors： Jeon SI,Hong JW,Yoon HC

更新日期：2006-09-01 00:00:00

abstract：:A novel gene encoding the α-chain of goose immunoglobulin heavy-chain (Igα) was cloned by reverse transcription-PCR. The cDNA had 1,760 bp and encompassed a partial V-D-J region of the heavy chain, a constant region (Cα) and 3'-untranslated region of α-chain. The Cα gene contains four constant region domains (CH1-CH4)...

journal_title：Biotechnology letters

authors： Guo Y,Gao M,Luo X,Sheng Q,Ma B,Wang J

更新日期：2014-04-01 00:00:00

abstract：:Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...

journal_title：Biotechnology letters

authors： Rahman M,Hasan MR,Shimizu K

更新日期：2008-05-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study is to report a ι-carrageenase which degrades ι-carrageenan yielding neo-ι-carratetraose as the main product in the absence of NaCl. The gene for a new ι-carrageenase, CgiB_Ce, from Cellulophaga sp. QY3 was cloned and sequenced. It comprised an ORF of 1,386 bp encoding for a pro...

journal_title：Biotechnology letters

authors： Ma S,Tan YL,Yu WG,Han F

更新日期：2013-10-01 00:00:00

abstract：:Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...

journal_title：Biotechnology letters

authors： Gao J,Zhang Y,Zhang W,Wu S,Qin S,Zhang W,Yu X

更新日期：2005-09-01 00:00:00

abstract：:Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...

journal_title：Biotechnology letters

authors： Shah N,Anwar S,Xu J,Hou Z,Salah A,Khan S,Gong J,Shang Z,Qian L,Zhang C

更新日期：2018-08-01 00:00:00

abstract：:Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, ...

journal_title：Biotechnology letters

authors： Dong XY,Xiu ZL,Li S,Hou YM,Zhang DJ,Ren CS

更新日期：2010-09-01 00:00:00

abstract：OBJECTIVE:To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS:A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked t...

journal_title：Biotechnology letters

authors： Zhang S,Huang J,Hu R,Guo G,Shang X,Wu J

更新日期：2017-08-01 00:00:00

abstract：:Degradation of lignocellulose with pressurised hot water is an efficient method of bioethanol production. However, the resultant solution inhibits ethanol fermentation by Saccharomyces cerevisiae. Here, we first report that glycolaldehyde, which is formed when lignocellulose is treated with pressurised hot water, inhi...

journal_title：Biotechnology letters

authors： Jayakody LN,Hayashi N,Kitagaki H

更新日期：2011-02-01 00:00:00

abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily by enzymic modification of these drugs. One important mechanism of streptomycin modification is through ATP-dependent O-adenylation, catalyzed by streptomycin adenylyltransferase. Initial velocity patterns deduced from steady state kinetics...

journal_title：Biotechnology letters

authors： Jana S,Deb JK

更新日期：2005-04-01 00:00:00

abstract：:By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pa...

journal_title：Biotechnology letters

authors： Abad S,Nahalka J,Winkler M,Bergler G,Speight R,Glieder A,Nidetzky B

更新日期：2011-03-01 00:00:00

abstract：:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...

journal_title：Biotechnology letters

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2006-05-01 00:00:00

abstract：:A gene encoding a gamma-butyrolactone autoregulator receptor was cloned in to E. coli from Streptomyces ambofaciens producing spiramycin, a macrolide antibiotic used in both veterinary medicine and human medicine. A 714-bp intact receptor gene (saaR) was obtained by PCR and genomic Southern hybridization with the 100-...

journal_title：Biotechnology letters

authors： Choi SU,Kim MK,Ha HS,Hwang YI

更新日期：2008-05-01 00:00:00

abstract：:Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...

journal_title：Biotechnology letters

authors： Morales M,Velázquez E,Jan J,Revah S,González U,Razo-Flores E

更新日期：2004-02-01 00:00:00

abstract：:The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, a...

journal_title：Biotechnology letters

authors： Shintani M,Fukushima N,Tezuka M,Yamane H,Nojiri H

更新日期：2008-01-01 00:00:00

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

journal_title：Biotechnology letters

authors： Adamitsch BF,Karner F,Hampel WA

更新日期：2003-05-01 00:00:00

abstract：:Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...

journal_title：Biotechnology letters

authors： Grabar TB,Zhou S,Shanmugam KT,Yomano LP,Ingram LO

更新日期：2006-10-01 00:00:00

abstract：:Laccase was produced by Coriolopsis rigida using barley bran as substrate in solid-state fermentation (SSF) and also by submerged fermentation (SmF). The best results were obtained in SSF with twice the amount of laccase production. Laccase could be produced from repeated batch cultures of SSF over 30 days. The laccas...

journal_title：Biotechnology letters

authors： Gómez J,Rodríguez Solar D,Pazos M,Sanromán MA

更新日期：2006-07-01 00:00:00

abstract：OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...

journal_title：Biotechnology letters

authors： Tardy GM,Lóránt B,Lóka M,Nagy B,László K

更新日期：2017-07-01 00:00:00

abstract：OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...

journal_title：Biotechnology letters

authors： Shariati L,Modaress M,Khanahmad H,Hejazi Z,Tabatabaiefar MA,Salehi M,Modarressi MH

更新日期：2016-08-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...

journal_title：Biotechnology letters

authors： Ge B,Tang Z,Lin L,Ren Y,Yang Y,Qin S

更新日期：2005-06-01 00:00:00

abstract：:Feruloylated mono- and di-acylglycerols were synthesized in a two step reaction: ethyl ferulate was first transesterified with glycerol and then this was esterified with oleic acid. The yield of the combined feruloylated mono- and di-acylglycerols in the second reaction reached 96% when esterification of 0.3 g transes...

journal_title：Biotechnology letters

authors： Sun S,Shan L,Liu Y,Jin Q,Wang X,Wang Z

更新日期：2007-12-01 00:00:00

abstract：:Centaurea calcitrapa suspension cultures were grown either in Erlenmeyer flasks or in a mechanically stirred bioreactor. Its rheological behaviour, when fitted to the Oswald-de Waele model (power law), showed pseudoplastic characteristics in both cases. The flow behaviour index (n) decreased over the course of a growt...

journal_title：Biotechnology letters

authors： Raposo S,Lima-Costa ME

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization o...

journal_title：Biotechnology letters

authors： Holtmann D,Mangold KM,Schrader J

更新日期：2009-05-01 00:00:00

abstract：:Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...

journal_title：Biotechnology letters

authors： Clauwaert P,Desloover J,Shea C,Nerenberg R,Boon N,Verstraete W

更新日期：2009-10-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00