Abstract:
:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H22 hepatoma (p ( 0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Ge B,Tang Z,Lin L,Ren Y,Yang Y,Qin Sdoi
10.1007/s10529-005-5794-7keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
783-7issue
11eissn
0141-5492issn
1573-6776journal_volume
27pub_type
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更新日期:2013-08-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2004-12-01 00:00:00
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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更新日期:2015-02-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2018-01-01 00:00:00