Abstract:
:Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species and one filamentous fungus were recorded as in vitro contaminants, whereas five yeast species were isolated from the field material. The bacterium, Pseudomonas reactans (6 isolates), and the yeast, Torulaspora delbrueckii (8 isolates), were the most frequent in vitro contaminants. Most of the bacterial species isolated from in vitro enset were Gram-positive and hitherto unrecorded as in vitro contaminants. The difficulty in controlling the in vitro contaminants is due to their apparent endogenous nature and their resistance to antimicrobial drugs.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Birmeta G,Passoth V,Roos S,Welander Mdoi
10.1007/s10529-004-6032-4keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1867-72issue
24eissn
0141-5492issn
1573-6776journal_volume
26pub_type
杂志文章abstract::A new aqueous two phase system comprising polyethylene glycol and xanthan is reported together with its phase diagram. The phase composition of the bottom phase did not vary (PEG 1.6-1.8% w/w; xanthan 0.24-0.28% w/w) while that of the top phase varied significantly (PEG 4-5% w/w, xanthan 0.05-1.37% w/w). Unlike conven...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4682-5
更新日期:2006-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
abstract::There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical prof...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0108-0
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00
abstract::A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9661-1
更新日期:2008-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
更新日期:2003-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0404-8
更新日期:2011-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0650-4
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:An extracellular protease inhibitor (BTPI-301) of trypsin was purified and characterized from an isolate of Pseudomonas mendocina. RESULTS:BTPI-301was purified to homogeneity by (NH4)2SO4, precipitation, DEAE Sepharose and CNBr-activated Sepharose chromatography. Homogeneity was proved by native PAGE and SD...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2424-0
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02850-z
更新日期:2020-06-01 00:00:00
abstract::Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4951-3
更新日期:2006-01-01 00:00:00
abstract::Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0048-8
更新日期:2009-10-01 00:00:00
abstract::The enantioselective hydrolysis of insoluble (R,S)-ketoprofen ethyl ester to the optically active (S)-ketoprofen was carried out in a dispersed aqueous lipase reaction system induced by the inclusion of chiral cyclodextrins for complexation of the substrate. Hydroxypropyl-beta-cyclodextrin was the most effective chira...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000030040.13828.d7
更新日期:2004-06-01 00:00:00
abstract::Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1350-z
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:The promoter of HpMET3, encoding an ATP sulfurylase, was evaluated for its potential as a repressible promoter to downregulate the expression of target genes in the thermotolerant, methylotrophic yeast Hansenula polymorpha. RESULTS:The expression of lacZ under the control of the 0.6 kb HpMET3 promoter was e...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1902-5
更新日期:2015-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1467-8
更新日期:2014-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1670-7
更新日期:2015-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
abstract::The impact of the two adaptation-induced mutations in an improved xylose-fermenting Zymomonas mobilis strain was investigated. The chromosomal mutation at the xylose reductase gene was critical to xylose metabolism by reducing xylitol formation. Together with the plasmid-borne mutation impacting xylose isomerase activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0970-z
更新日期:2012-10-01 00:00:00
abstract::Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9651-3
更新日期:2008-06-01 00:00:00
abstract::We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0544-5
更新日期:2011-06-01 00:00:00
abstract:OBJECTS:To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients. RESULTS:GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2077-4
更新日期:2016-06-01 00:00:00
abstract::Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1596-0
更新日期:2014-11-01 00:00:00
abstract::Long-term cell recycle fermentations of Candida tropicalis were performed over 14 rounds of fermentation. The average xylitol concentrations, fermentation times, volumetric productivities and product yields for 14 rounds were 105 g l(-1), 333 h, 4.4 g l(-1) h(-1) and 78%, respectively, in complex medium; and 110 g l(-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000023019.02411.54
更新日期:2004-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0985-5
更新日期:2012-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
abstract::For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein production and ammonia accumulation in transiently transfected CHO a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0809-z
更新日期:2012-04-01 00:00:00