Maintenance of human adipose derived stem cell (hASC) differentiation capabilities using a 3D culture.

abstract：:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The dete...

journal_title：Biotechnology letters

authors： Qiao YM,Guo YC,Zhang XE,Zhou YF,Zhang ZP,Wei HP,Yang RF,Wang DB

更新日期：2007-12-01 00:00:00

abstract：:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...

journal_title：Biotechnology letters

authors： Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková B

更新日期：2008-04-01 00:00:00

abstract：OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...

journal_title：Biotechnology letters

authors： Stepanov A,Belyy A,Kasheverov I,Rybinets A,Dronina M,Dyachenko I,Murashev A,Knorre V,Sakharov D,Ponomarenko N,Tsetlin V,Tonevitsky A,Deyev S,Belogurov A Jr,Gabibov A

更新日期：2016-07-01 00:00:00

abstract：:Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...

journal_title：Biotechnology letters

authors： Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Y

更新日期：2020-12-01 00:00:00

abstract：:Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...

journal_title：Biotechnology letters

authors： Villanueva-Alonzo HJ,Us-Camas RY,López-Ochoa LA,Robertson D,Guerra-Peraza O,Minero-García Y,Moreno-Valenzuela OA

更新日期：2013-05-01 00:00:00

abstract：:A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...

journal_title：Biotechnology letters

authors： Woo JH,Kwon TH,Kim JT,Kim CG,Lee EY

更新日期：2013-04-01 00:00:00

abstract：OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...

journal_title：Biotechnology letters

authors： Wang W,Yu Y,Dou TY,Wang JY,Sun C

更新日期：2018-02-01 00:00:00

abstract：:This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...

journal_title：Biotechnology letters

authors： Hildén L,Johansson G

更新日期：2004-11-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6 in the lentiviral transduction. The lentiviral vector was use...

journal_title：Biotechnology letters

authors： Picanço-Castro V,Fontes AM,Heinz S,Tonn T,Covas DT

更新日期：2008-02-01 00:00:00

abstract：OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...

journal_title：Biotechnology letters

authors： Tardy GM,Lóránt B,Lóka M,Nagy B,László K

更新日期：2017-07-01 00:00:00

abstract：OBJECTIVES:To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. RESULTS:The elongation factor P (EF-P) promoter from Bifidobacterium...

journal_title：Biotechnology letters

authors： Montenegro-Rodríguez C,Peirotén A,Sanchez-Jimenez A,Arqués JL,Landete JM

更新日期：2015-07-01 00:00:00

abstract：:Phanerochaete chrysoporium morphology and manganese peroxidase (MnP) productivity was characterised in a scalable, modularised 1145 cm(3) membrane gradostat reactor in response to switching between an enhanced production medium and a nutrient limited feed (50% C and N reduction). Irrespective of the feed composition u...

journal_title：Biotechnology letters

authors： Govender S

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, ...

journal_title：Biotechnology letters

authors： Dong XY,Xiu ZL,Li S,Hou YM,Zhang DJ,Ren CS

更新日期：2010-09-01 00:00:00

abstract：:Semliki forest virus (SFV) is a pathogen causing lethal encephalitis in laboratory mice. In this study, we obtained three short hairpin RNAs (shRNAs) which could specifically target SFV sequence in GFP reporting systems and effectively suppress SFV replication in luciferase-containing reporter virus system. At a multi...

journal_title：Biotechnology letters

authors： Zhang K,Chen Y,Pan J,Ahola T,Guo D

更新日期：2009-04-01 00:00:00

abstract：:To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...

journal_title：Biotechnology letters

authors： Yuan XF,Dai ZH,Wang XD,Zhao B

更新日期：2009-08-01 00:00:00

abstract：:Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...

journal_title：Biotechnology letters

authors： van Eijsden RG,Stassen C,Daenen L,Van Mulders SE,Bapat PM,Siewers V,Goossens KV,Nielsen J,Delvaux FR,Van Hummelen P,Devreese B,Willaert RG

更新日期：2013-06-01 00:00:00

abstract：:Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...

journal_title：Biotechnology letters

authors： Davolos CC,Guidelli-Thuler AM,de Abreu IL,Sena JA,Lemos MV

更新日期：2009-12-01 00:00:00

abstract：:Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...

journal_title：Biotechnology letters

authors： Trung TS,Ng CH,Stevens WF

更新日期：2003-07-01 00:00:00

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：:Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...

journal_title：Biotechnology letters

authors： Larson TM,Anderson AM,Rich JO

更新日期：2013-02-01 00:00:00

abstract：:Biotechnology using molecular biology, biochemistry, biophysics, and computational approaches provides an alternative approach for classical pharmacological screening to look at ligand-receptor interactions and receptor specificity, which should support the design of selective drugs based on detailed structural princi...

journal_title：Biotechnology letters

authors： Ratnala VR

更新日期：2006-06-01 00:00:00

abstract：:Targeted gene replacement in the thermotolerant yeast Kluyveromyces marxianus KCTC 17555 has been hampered by its propensity to non-homologous end joining (NHEJ). To enhance homologous recombination (HR) by blocking NHEJ, we identified and disrupted the K. marxianus KU80 gene. The ku80 deletion mutant strain (Kmku80∆)...

journal_title：Biotechnology letters

authors： Choo JH,Han C,Kim JY,Kang HA

更新日期：2014-10-01 00:00:00

abstract：:The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...

journal_title：Biotechnology letters

authors： Wu JR,Lin Y,Zheng ZY,Lin CC,Zhan XB,Shen YQ

更新日期：2010-12-01 00:00:00

abstract：OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-sp...

journal_title：Biotechnology letters

authors： Chang Y,Shao J,Gao Y,Liu W,Gao Z,Hu Y,Chang H

更新日期：2020-08-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened ...

journal_title：Biotechnology letters

authors： Korekar G,Sharma RK,Kumar R,Meenu,Bisht NC,Srivastava RB,Ahuja PS,Stobdan T

更新日期：2012-05-01 00:00:00

abstract：:By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsuppleme...

journal_title：Biotechnology letters

authors： Chen PT,Chao YP

更新日期：2006-10-01 00:00:00

abstract：:Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using ...

journal_title：Biotechnology letters

authors： Kim YM,Kim BH,Ahn JS,Kim GE,Jin SD,Nguyen TH,Kim D

更新日期：2009-09-01 00:00:00