Abstract:
:A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its derivatives, such as 2,3- and 4-chlorostyrene oxides, as a sole carbon source. Gordonia sp. H37 exhibited high enantioselective hydrolysis activity for 4-chlorostyrene oxide with an enantiomeric ratio of 27. Gordonia sp. H37 preferentially hydrolyzed the (R)-enantiomer of styrene oxide derivatives resulting in the preparation of a (S)-enantiomer with enantiomeric excess greater than 99.9 %. The enantioselective EHase activity was identified and characterized in various PAH-degrading bacteria, and whole cell Gordonia sp. H37 was employed as a biocatalyst for preparing enantiopure (S)-styrene oxide derivatives.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Woo JH,Kwon TH,Kim JT,Kim CG,Lee EYdoi
10.1007/s10529-012-1114-1subject
Has Abstractpub_date
2013-04-01 00:00:00pages
599-606issue
4eissn
0141-5492issn
1573-6776journal_volume
35pub_type
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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pub_type: 杂志文章
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pub_type: 杂志文章
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