Abstract:
OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-specific knock-in into Marc-145 cells by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and putatively explored appropriate genomic recombination sites in Marc-145 cells. RESULTS:Our study demonstrated that the specific homologous recombination (HR) site between the Rac GTPase activating protein 1 (RACGAP1) and the acid-sensing ion channel subunit 1 (ASIC1) genes of the 11th chromosome could be used as the target site of Cas9 for the generation of target gene knock-in into Marc-145 cells, by the insertion of the eGFP cassette into the specific HR site and subsequent expression. CONCLUSIONS:Junction PCR, sequencing, Southern blot and fluorescence assay determined eGFP gene-specific knock-in HR site between the RACGAP1 and ASIC1 genes of the 11th chromosome, which was identified by the genomic safe harbours in Marc-145 cells. Our study encouraged a broader range of applications, such as Marc-145 cells development and engineering for virus adaption and yield increase in the vaccine biotechnology industry.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Chang Y,Shao J,Gao Y,Liu W,Gao Z,Hu Y,Chang Hdoi
10.1007/s10529-020-02860-xsubject
Has Abstractpub_date
2020-08-01 00:00:00pages
1317-1325issue
8eissn
0141-5492issn
1573-6776pii
10.1007/s10529-020-02860-xjournal_volume
42pub_type
杂志文章abstract:OBJECTIVES:To improve the production of α-ketoglutaric acid (α-KG) from L-glutamate by whole-cell biocatalysis. RESULTS:A novel and highly active L-glutamate oxidase, SmlGOX, from Streptomyces mobaraensis was overexpressed and purified. The recombinant SmlGOX was approx. 64 kDa by SDS-PAGE. SmlGOX had a maximal activi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2314-5
更新日期:2017-06-01 00:00:00
abstract::An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0568-x
更新日期:2011-07-01 00:00:00
abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025016221891
更新日期:2003-08-01 00:00:00
abstract::The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0024-3
更新日期:2009-09-01 00:00:00
abstract::To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1489-2
更新日期:2014-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2631-3
更新日期:2019-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2123-2
更新日期:2016-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9528-x
更新日期:2008-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2191-3
更新日期:2016-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract::O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0178-z
更新日期:2010-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000007079.13308.2f
更新日期:2003-12-01 00:00:00
abstract::The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed und...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9280-7
更新日期:2007-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-3185-0
更新日期:2004-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9821-3
更新日期:2008-12-01 00:00:00
abstract::Feruloylated mono- and di-acylglycerols were synthesized in a two step reaction: ethyl ferulate was first transesterified with glycerol and then this was esterified with oleic acid. The yield of the combined feruloylated mono- and di-acylglycerols in the second reaction reached 96% when esterification of 0.3 g transes...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9486-3
更新日期:2007-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9683-8
更新日期:2008-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0269-x
更新日期:2010-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9533-0
更新日期:2008-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0001-x
更新日期:2009-08-01 00:00:00
abstract::In the industrial production of bioethanol from lignocellulosic biomass, a strain of Saccharomyces cerevisiae that can ferment xylose in the presence of inhibitors is of utmost importance. The recombinant, industrial-flocculating S. cerevisiae strain NAPX37, which can ferment xylose, was used as the parent to delete t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1581-7
更新日期:2014-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2450-y
更新日期:2018-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02745-8
更新日期:2019-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0883-x
更新日期:2012-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1335-y
更新日期:2014-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1244-0
更新日期:2013-10-01 00:00:00
abstract::Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1228-0
更新日期:2013-09-01 00:00:00
abstract::A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0404-8
更新日期:2011-01-01 00:00:00
abstract::Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6934-1
更新日期:2005-01-01 00:00:00