Green asymmetric reduction of acetophenone derivatives: Saccharomyces cerevisiae and aqueous natural deep eutectic solvent.

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：:Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...

journal_title：Biotechnology letters

authors： Wu J,Zhang HB,Wang J,Yang TC,Xian J,Yang CH,Lai HW

更新日期：2008-06-01 00:00:00

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...

journal_title：Biotechnology letters

authors： Trujillo-Villanueva K,Rubio-Piña J,Monforte-González M,Ramírez-Benítez E,Vázquez-Flota F

更新日期：2012-02-01 00:00:00

abstract：:A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...

journal_title：Biotechnology letters

authors： Zhou WW,Niu TG

更新日期：2009-01-01 00:00:00

abstract：:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...

journal_title：Biotechnology letters

authors： Ge B,Tang Z,Lin L,Ren Y,Yang Y,Qin S

更新日期：2005-06-01 00:00:00

abstract：:The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various...

journal_title：Biotechnology letters

authors： Park JH,Song MH,Lee CH,Lee MK,Park YM,Old L,Lee SY

更新日期：2011-06-01 00:00:00

abstract：:Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...

journal_title：Biotechnology letters

authors： Clauwaert P,Desloover J,Shea C,Nerenberg R,Boon N,Verstraete W

更新日期：2009-10-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...

journal_title：Biotechnology letters

authors： Woo JH,Kwon TH,Kim JT,Kim CG,Lee EY

更新日期：2013-04-01 00:00:00

abstract：:The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...

journal_title：Biotechnology letters

authors： Wu JR,Lin Y,Zheng ZY,Lin CC,Zhan XB,Shen YQ

更新日期：2010-12-01 00:00:00

abstract：:Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure ...

journal_title：Biotechnology letters

authors： Jang J,Moon SJ,Hong SH,Kim IH

更新日期：2010-11-01 00:00:00

abstract：:L-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while...

journal_title：Biotechnology letters

authors： Roble ND,Ogbonna JC,Tanaka H

更新日期：2003-07-01 00:00:00

abstract：OBJECTIVES:To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. RESULTS:The fungi were cultivated on wheat bran and the resulting supernatants were combined with Cellucl...

journal_title：Biotechnology letters

authors： Peciulyte A,Pisano M,de Vries RP,Olsson L

更新日期：2017-09-01 00:00:00

abstract：:The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...

journal_title：Biotechnology letters

authors： West TP

更新日期：2011-12-01 00:00:00

abstract：OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we f...

journal_title：Biotechnology letters

authors： Huang X,Zheng M,Yi Y,Patel A,Song Z,Li Y

更新日期：2020-11-01 00:00:00

abstract：:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...

journal_title：Biotechnology letters

authors： Glendinning KJ,Macaskie LE,Brown NL

更新日期：2005-11-01 00:00:00

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00

abstract：OBJECTIVE:To characterize the hydrolysis product and the substrate binding in the catalytic cavity of α-agarase AgaD. RESULTS:The time course curve showed that AgaD degraded agarose by the endo-type cleavage. AgaD did not degrade agarobiose (A2) and agarotetraose (A4). The minimum-length substrate was agarohexaose (A6...

journal_title：Biotechnology letters

authors： Wang H,Zhang W,Cui Z,Lu Z,Lu X

更新日期：2020-10-01 00:00:00

abstract：:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...

journal_title：Biotechnology letters

authors： Tounsi S,Jaoua S

更新日期：2003-08-01 00:00:00

abstract：:Growth and sporulation of Verticillium lecanii on inert and organic carriers (sugar-cane bagasse, corncob, rice straw, polyurethane foam and activated carbon) in a solid-state fermentation process was studied. Sugar-cane bagasse and polyurethane foam produced 10(10) spores g(-1) dry carrier whereas corncob, rice straw...

journal_title：Biotechnology letters

authors： Xu X,Yu Y,Shi Y

更新日期：2011-04-01 00:00:00

abstract：:KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...

journal_title：Biotechnology letters

authors： Liu Y,Ren L,Ge L,Cui Q,Cao X,Hou Y,Bai F,Bai G

更新日期：2014-08-01 00:00:00

abstract：:O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

journal_title：Biotechnology letters

authors： Ning H,Zhang C,Yao Y,Yu D

更新日期：2010-04-01 00:00:00

abstract：:Baker's yeast suspensions were incubated at different pressures (from 1 bar to 6 bar) and different gases [air, O(2) and a mixture of 8% (v/v) CO(2), 21% O(2) and N(2)]. Raising the air pressure from 1 bar to 6 bar stimulated cell growth but had no effect on leavening ability or viability of the cells. A 50% reduction...

journal_title：Biotechnology letters

authors： Campelo AF,Belo I

更新日期：2004-08-01 00:00:00

abstract：:A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...

journal_title：Biotechnology letters

authors： Pöhnlein M,Finkbeiner T,Syldatk C,Hausmann R

更新日期：2015-03-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：:Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of ar...

journal_title：Biotechnology letters

authors： Lee DW,Baney R

更新日期：2004-05-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a ...

journal_title：Biotechnology letters

authors： Karasaki N,Mon H,Takahashi M,Lee JM,Koga K,Kawaguchi Y,Kusakabe T

更新日期：2009-04-01 00:00:00