The sequential exposure to jasmonate, salicylic acid and yeast extract promotes sanguinarine accumulation in Argemone mexicana cell cultures.

abstract：OBJECTIVES:The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. RESULTS:EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of s...

journal_title：Biotechnology letters

authors： Yu M,Qiu Y,Chen W,Zhao F,Shao J

更新日期：2015-08-01 00:00:00

abstract：:N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...

journal_title：Biotechnology letters

authors： Yang SR,Kim SB,Joe CO,Kim JD

更新日期：2005-07-01 00:00:00

abstract：:ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial...

journal_title：Biotechnology letters

authors： Shen Y,Huang H,Zhu L,Luo M,Chen D

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro. RESULTS:By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-ti...

journal_title：Biotechnology letters

authors： Zhao Y,Wang T,Yu Z,Wang H,Liu B,Wu C,Teng CB

更新日期：2016-04-01 00:00:00

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...

journal_title：Biotechnology letters

authors： Fouladiha H,Marashi SA,Li S,Li Z,Masson HO,Vaziri B,Lewis NE

更新日期：2021-01-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:A polysaccharide from the traditional Chinese medicinal herb, Bletilla striata (Thunb.) Reichb. f., was isolated, purified and characterized. It induced the proliferation of human umbilical vascular endothelial cells and the expression of vascular endothelial growth factor up to 156% and 147% of control after 72 h, re...

journal_title：Biotechnology letters

authors： Wang C,Sun J,Luo Y,Xue W,Diao H,Dong L,Chen J,Zhang J

更新日期：2006-04-01 00:00:00

abstract：:To explore the feasibility of larch (Larix decidua Mill.) embryogenic cell culture as alternative plant expression system, protein stability in fresh and conditioned medium was characterized in this study and compared to tobacco BY2 suspension culture. Fresh and conditioned media were spiked with 1 microg human IgG an...

journal_title：Biotechnology letters

authors： Calinski A,Classen B,Zoglauer K,Boehm R

更新日期：2009-05-01 00:00:00

abstract：:Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...

journal_title：Biotechnology letters

authors： Zhao J,Han Y,Wang Z,Zhang R,Wang G,Mao Y

更新日期：2020-12-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...

journal_title：Biotechnology letters

authors： Johnson EE,Jetter R,Wasteneys G

更新日期：2014-04-01 00:00:00

abstract：:Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...

journal_title：Biotechnology letters

authors： Wu XB,Tian XY,Ji JJ,Wu WB,Fan KQ,Yang KQ

更新日期：2011-04-01 00:00:00

abstract：:Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...

journal_title：Biotechnology letters

authors： Chung CP,Jang JH

更新日期：2005-06-01 00:00:00

abstract：:A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO ...

journal_title：Biotechnology letters

authors： Yang Y,Wang J,Bu D,Zhang L,Li S,Zhou L,Wei H

更新日期：2011-01-01 00:00:00

abstract：:Batch fermentation by Streptomyces pristinaespiralis with the addition of adsorbent resins was used to increase the production of pristinamycin. In consideration of the adsorption capacity and the desorption ability, a polymeric resin, JD-1, was finally selected. The maximum production of pristinamycin in Erlenmeyer f...

journal_title：Biotechnology letters

authors： Jia B,Jin ZH,Lei YL,Mei LH,Li NH

更新日期：2006-11-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：:The enantioselective hydrolysis of insoluble (R,S)-ketoprofen ethyl ester to the optically active (S)-ketoprofen was carried out in a dispersed aqueous lipase reaction system induced by the inclusion of chiral cyclodextrins for complexation of the substrate. Hydroxypropyl-beta-cyclodextrin was the most effective chira...

journal_title：Biotechnology letters

authors： Kim SH,Kim TK,Shin GS,Lee KW,Shin HD,Lee YH

更新日期：2004-06-01 00:00:00

abstract：:The expression of the gene OsITL1 coding for the inositol 1,3,4-trisphosphate 5/6-kinase protein was induced by 200 mM NaCl or dehydration. The expression of OsITL1 in response to NaCl and dehydration suggests the possible functions of OsITL1 in osmotic stress responses; however, physiological tests indicate that the ...

journal_title：Biotechnology letters

authors： Niu X,Chen Q,Wang X

更新日期：2008-09-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00

abstract：OBJECTIVE:To produce (S)-3-hydroxy-1-(3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-4-(2,4,5-trifluorophenyl)butan-1-one (S)-1 from 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)-1-(2,4,5-trifluorophenyl)butan-2-one (2) by microbial bioreduction. RESULTS:A new...

journal_title：Biotechnology letters

authors： Wei Y,Xia S,He C,Xiong W,Xu H

更新日期：2016-05-01 00:00:00

abstract：OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

journal_title：Biotechnology letters

authors： Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

更新日期：2021-01-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) is a heterodimer containing 66 kDa p66 and 51 kDa p51 subunits. We previously showed that HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT have higher affinities for dTTP and template-primer (T/P) than Moloney murine leukemia virus RT, whi...

journal_title：Biotechnology letters

authors： Nishimura K,Shinomura M,Konishi A,Yasukawa K

更新日期：2013-12-01 00:00:00

abstract：:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...

journal_title：Biotechnology letters

authors： Kuan IC,Chuang CA,Lee SL,Yu CY

更新日期：2012-08-01 00:00:00

abstract：:Four Cr(VI)-reducing bacterial strains (Ochrobactrum intermedium, CrT-2, CrT-3 and CrT-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (Helianthus annuus var SF-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg CrCl...

journal_title：Biotechnology letters

authors： Faisal M,Hasnain S

更新日期：2005-07-01 00:00:00

abstract：:Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette. Evidence ...

journal_title：Biotechnology letters

authors： Peng H,Fu B,Mao Z,Shao W

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVES:To prepare (R)-phenyl-1,2-ethanediol ((R)-PED) with high enantiomeric excess (ee p) and yield from racemic styrene oxide (rac-SO) at high concentration by bi-enzymatic catalysis. RESULTS:The bi-enzymatic catalysis was designed for enantioconvergent hydrolysis of rac-SO by a pair of novel epoxide hydrolases ...

journal_title：Biotechnology letters

authors： Wang R,Hu D,Zong X,Li J,Ding L,Wu M,Li J

更新日期：2017-12-01 00:00:00

abstract：:A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...

journal_title：Biotechnology letters

authors： Park CS,Kim JE,Lee SH,Kim YS,Kang LW,Oh DK

更新日期：2013-11-01 00:00:00

abstract：:The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...

journal_title：Biotechnology letters

authors： Park HY,Kim DH,Sivanesan I

更新日期：2017-09-01 00:00:00