Abstract:
:The expression of the gene OsITL1 coding for the inositol 1,3,4-trisphosphate 5/6-kinase protein was induced by 200 mM NaCl or dehydration. The expression of OsITL1 in response to NaCl and dehydration suggests the possible functions of OsITL1 in osmotic stress responses; however, physiological tests indicate that the expression of OsITL1 in tobacco decreases tolerance to NaCl during germination and seedling development. This result was consistent with that obtained on treatment of mature tobacco seedlings with NaCl (200 mM), suggesting that OsITL1 inversely regulates plant responses to osmotic stress.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Niu X,Chen Q,Wang Xdoi
10.1007/s10529-008-9730-5subject
Has Abstractpub_date
2008-09-01 00:00:00pages
1687-92issue
9eissn
0141-5492issn
1573-6776journal_volume
30pub_type
杂志文章abstract:OBJECTIVE:To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production. RESULTS:A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1957-3
更新日期:2016-01-01 00:00:00
abstract::Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1350-z
更新日期:2014-02-01 00:00:00
abstract::The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02920-2
更新日期:2020-10-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9013-y
更新日期:2006-06-01 00:00:00
abstract::Rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. In all cultures, a carbohydrate polymer fraction remained resistant to fermentation. These fractions were isolated in gram amounts and characterised. The molecular weight di...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000003975.28654.6e
更新日期:2003-11-01 00:00:00
abstract::In vitro synthesized mRNAs are often used as calibrants in gene expression analysis. In the case of an analytical procedure for gene expression containing a process of reverse transcription such as microarray analysis, full-length mRNAs having a 3'-poly(A)+tail are required as calibrants. However, the preparation of f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9847-6
更新日期:2009-02-01 00:00:00
abstract::Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
abstract::Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette. Evidence ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9184-6
更新日期:2006-12-01 00:00:00
abstract::Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0015-4
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2175-3
更新日期:2016-11-01 00:00:00
abstract::The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1590-6
更新日期:2014-11-01 00:00:00
abstract::Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025072020791
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVES:To improve target protein production by manipulating expression levels of alanine racemase in Bacillus licheniformis. RESULTS:The gene of dal was identified to be responsible for alanine racemase function. Based on the selection marker of dal, a food-grade expression system was constructed in B. licheniform...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2359-5
更新日期:2017-09-01 00:00:00
abstract::Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0741-2
更新日期:2012-01-01 00:00:00
abstract::In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2218-9
更新日期:2017-01-01 00:00:00
abstract::Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0635-3
更新日期:2011-09-01 00:00:00
abstract::Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3903-2
更新日期:2005-12-01 00:00:00
abstract::A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9561-9
更新日期:2008-03-01 00:00:00
abstract::By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0456-9
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2523-6
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. RESULTS:Quantitative real-time PCR analysis indicated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2528-1
更新日期:2018-05-01 00:00:00
abstract::The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
abstract::Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1004-6
更新日期:2012-11-01 00:00:00
abstract::The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0720-7
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1824-2
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2385-3
更新日期:2017-10-01 00:00:00
abstract::The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024951322955
更新日期:2003-08-01 00:00:00
abstract::Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
abstract::The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7879-0
更新日期:2005-02-01 00:00:00