Abstract:
:Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activity was needed in the engineered E. coli when IPP activity was increased. The introduction of GGPS and IPI increased the β-carotene content in E. coli from 0.5 to 0.95 mg/g dry wt.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Sun J,Sun XX,Tang PW,Yuan QPdoi
10.1007/s10529-012-1004-6subject
Has Abstractpub_date
2012-11-01 00:00:00pages
2077-82issue
11eissn
0141-5492issn
1573-6776journal_volume
34pub_type
杂志文章abstract::We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9463-x
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1217-3
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1721-0
更新日期:2015-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0238-4
更新日期:2010-07-01 00:00:00
abstract::Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1355-7
更新日期:2014-02-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2007-02-01 00:00:00
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journal_title:Biotechnology letters
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1021-5
更新日期:2012-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2196-y
更新日期:2016-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023038804556
更新日期:2003-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/BF01026199
更新日期:1987-12-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
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doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1632-0
更新日期:2014-12-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2007-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2348-8
更新日期:2017-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0024-x
更新日期:2005-08-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2015-02-01 00:00:00
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pub_type: 历史文章,杂志文章,评审
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更新日期:2010-10-01 00:00:00
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更新日期:2020-09-01 00:00:00
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更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2013-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02850-z
更新日期:2020-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2081-8
更新日期:2016-07-01 00:00:00