High-level production of α-amylase by manipulating the expression of alanine racamase in Bacillus licheniformis.

abstract：:An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...

journal_title：Biotechnology letters

authors： Hwang SH,Koo YM

更新日期：2003-02-01 00:00:00

abstract：:Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...

journal_title：Biotechnology letters

authors： Castilho-Fernandes A,Fontes AM,Abraham KJ,de Freitas MC,da Rosa NG,Picanço-Castro V,de Sousa Russo-Carbolante EM,Covas DT

更新日期：2015-05-01 00:00:00

abstract：:Tempe was prepared using mixtures of natural soybean and genetically modified Roundup Ready (RUR) soybean fermented with natural Rhizopus sp. The amount of RUR soybean was quantified using an ELISA plate test. The RUR signal decreased during fermentation. In the control experiments on fermentation of non-RUR soybean, ...

journal_title：Biotechnology letters

authors： Prakoso B,Nitisinprasert S,Stevens WF

更新日期：2003-09-01 00:00:00

abstract：OBJECTIVES:To prepare (R)-phenyl-1,2-ethanediol ((R)-PED) with high enantiomeric excess (ee p) and yield from racemic styrene oxide (rac-SO) at high concentration by bi-enzymatic catalysis. RESULTS:The bi-enzymatic catalysis was designed for enantioconvergent hydrolysis of rac-SO by a pair of novel epoxide hydrolases ...

journal_title：Biotechnology letters

authors： Wang R,Hu D,Zong X,Li J,Ding L,Wu M,Li J

更新日期：2017-12-01 00:00:00

abstract：:Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...

journal_title：Biotechnology letters

authors： Smit MS,Mokgoro MM,Setati E,Nicaud JM

更新日期：2004-05-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...

journal_title：Biotechnology letters

authors： Zhu L,Cui W,Fang Y,Liu Y,Gao X,Zhou Z

更新日期：2013-05-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：:Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express...

journal_title：Biotechnology letters

authors： Seo HM,Jung Y,Song S,Kim Y,Kwon T,Kim DH,Jeung SJ,Yi YB,Yi G,Nam MH,Nam J

更新日期：2008-10-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：:A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could...

journal_title：Biotechnology letters

authors： Nakamura M,Mie M,Funabashi H,Kobatake E

更新日期：2004-07-01 00:00:00

abstract：:Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...

journal_title：Biotechnology letters

authors： Redwood MD,Dhillon R,Orozco RL,Zhang X,Binks DJ,Dickinson M,Macaskie LE

更新日期：2012-12-01 00:00:00

abstract：:UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation o...

journal_title：Biotechnology letters

authors： Liu W,Lu X,He G,Gao X,Li M,Wu J,Li Z,Wu J,Wang J,Luo C

更新日期：2013-04-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA(3), was integrated into L. ...

journal_title：Biotechnology letters

authors： Wang L,Li X,Chen S,Liu G

更新日期：2009-02-01 00:00:00

abstract：:The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone (PQQGDH) was modified to greatly simplify its purification. The surface charge of PQQGDH was engineered by either fusing a three-arginine tail to the C-terminus of PQQGDH (PQQGDH+Arg3) or by substituting th...

journal_title：Biotechnology letters

authors： Koh H,Igarashi S,Sode K

更新日期：2003-10-01 00:00:00

abstract：:Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

journal_title：Biotechnology letters

authors： Li N,Zong MH,Ma D

更新日期：2009-08-01 00:00:00

abstract：:Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...

journal_title：Biotechnology letters

authors： Komaraiah P,Reddy GV,Reddy PS,Raghavendra AS,Ramakrishna SV,Reddanna P

更新日期：2003-04-01 00:00:00

abstract：:Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...

journal_title：Biotechnology letters

authors： Orlita A,Sidwa-Gorycka M,Malinski E,Czerwicka M,Kumirska J,Golebiowski M,Lojkowska E,Stepnowski P

更新日期：2008-03-01 00:00:00

abstract：OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolact...

journal_title：Biotechnology letters

authors： Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi T

更新日期：2021-01-26 00:00:00

abstract：:Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...

journal_title：Biotechnology letters

authors： Foster LJ,Catzel D,Atwa S,Zarka M,Mahler SM

更新日期：2003-08-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Mor...

journal_title：Biotechnology letters

authors： Tang M,Zhang RQ,Chen H,Zhang HH,Tian ZQ

更新日期：2008-10-01 00:00:00

abstract：:An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x...

journal_title：Biotechnology letters

authors： Aoyagi H

更新日期：2006-10-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) is a heterodimer containing 66 kDa p66 and 51 kDa p51 subunits. We previously showed that HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT have higher affinities for dTTP and template-primer (T/P) than Moloney murine leukemia virus RT, whi...

journal_title：Biotechnology letters

authors： Nishimura K,Shinomura M,Konishi A,Yasukawa K

更新日期：2013-12-01 00:00:00

abstract：OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...

journal_title：Biotechnology letters

authors： Cai Y,Hong HQ,Zhao L,Cai YP,Qin Y

更新日期：2015-10-01 00:00:00

abstract：:A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...

journal_title：Biotechnology letters

authors： Takagi M,Kitabayashi T,Koizumi S,Hirose H,Kondo S,Fujiwara M,Ueno K,Hiroaki M,Hosokawa Y,Masuhara H,Wakitani S

更新日期：2008-07-01 00:00:00

abstract：:A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp...

journal_title：Biotechnology letters

authors： Jin X,Feng D,Wang H,Wang J

更新日期：2007-09-01 00:00:00