Abstract:
OBJECTIVE:Heterotrophic manganese (Mn)-oxidizing microorganisms responsible for biogenic manganese oxide (Bio-MnOx) production are fastidious. Their enrichment is not easily accomplished by merely adding a soluble organic substrate to non-sterile mixed cultures. The objective of this study was to evaluate polycaprolactone (PCL), an aliphatic polyester, as an effective solid organic substrate for the enrichment of marine Mn-oxidizing microorganisms. RESULTS:We successfully obtained marine microbial enrichment with the capacity for dissolved Mn removal and MnOx production using PCL as a solid organic substrate. The removal of dissolved Mn by the Mn-oxidizing enrichment culture followed first-order kinetics with a rate constant of 0.014 h-1. 16S rRNA gene amplicon sequencing analysis revealed that the Mn-oxidizing enrichment culture was highly dominated by operational taxonomic units related to the bacterial phyla Cyanobacteria, Planctomycetes, and Proteobacteria. CONCLUSIONS:Our data demonstrate that PCL can serve as a potential substrate to enrich Mn-oxidizing microorganisms with the ability to produce MnOx under marine conditions.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Aoki M,Miyashita Y,Tran PT,Okuno Y,Watari T,Yamaguchi Tdoi
10.1007/s10529-021-03088-zsubject
Has Abstractpub_date
2021-01-26 00:00:00eissn
0141-5492issn
1573-6776pii
10.1007/s10529-021-03088-zpub_type
杂志文章abstract::Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...
journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022398917213
更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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