Abstract:
OBJECTIVES:To analyze the mechanisms underlying the impact of recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)-mediated BmK IT expression on the function of baculovirus GP64 envelope fusion protein and progeny virus production. RESULTS:Viral propagation assay indicated that overexpression GP64 could promote replication of AcMNPV. AcMNPV-mediated expression of BmK IT also promoted replication of AcMNPV. Immunofluorescence analysis showed BmK IT, which was regulated by very early promoter IE1 in AcMNPV, could make the GP64 protein move to the cytomembrane soon after transfection. BmK IT, which is regulated by P10 protein promoter (P10) and polyhedrosis promoter (PH), could promote the expression of GP64. CONCLUSION:BmK IT, regulated by very early promoter IE1, P10 protein promoter (P10) and PH, accelerated the expression of GP64 protein, promoted its early cytomembrane localization and then triggered virus budding and progeny virus production.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Fu Y,Miao Y,Zheng S,Du J,Liang Adoi
10.1007/s10529-016-2146-8subject
Has Abstractpub_date
2016-10-01 00:00:00pages
1673-81issue
10eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2146-8journal_volume
38pub_type
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