Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco.

abstract：:Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

journal_title：Biotechnology letters

authors： Li N,Zong MH,Ma D

更新日期：2009-08-01 00:00:00

abstract：:A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp...

journal_title：Biotechnology letters

authors： Jin X,Feng D,Wang H,Wang J

更新日期：2007-09-01 00:00:00

abstract：:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...

journal_title：Biotechnology letters

authors： Glendinning KJ,Macaskie LE,Brown NL

更新日期：2005-11-01 00:00:00

abstract：:Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...

journal_title：Biotechnology letters

authors： Noda K,Matsuno T,Fujii H,Kogure T,Urata M,Asami Y,Kuroda A

更新日期：2008-06-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...

journal_title：Biotechnology letters

authors： Tendulkar SR,Gupta A,Chattoo BB

更新日期：2003-11-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...

journal_title：Biotechnology letters

authors： Morales M,Velázquez E,Jan J,Revah S,González U,Razo-Flores E

更新日期：2004-02-01 00:00:00

abstract：OBJECTIVES:To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species. RESULTS:The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VP...

journal_title：Biotechnology letters

authors： Fujiwara R,Noda S,Kawai Y,Tanaka T,Kondo A

更新日期：2016-09-01 00:00:00

abstract：OBJECTIVES:This short commentary examines the factors that led to Food and Drug Administration's approval of the first plant-derived biologic. RESULTS:In 2012, the first plant-derived protein pharmaceutical (biologic) was approved for commercial use in humans. The product, a recombinant form of human β-glucocerebrosid...

journal_title：Biotechnology letters

authors： Mor TS

更新日期：2015-11-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：:An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...

journal_title：Biotechnology letters

authors： Zhu BW,Huang LS,Tan HD,Qin YQ,Du YG,Yin H

更新日期：2015-02-01 00:00:00

abstract：:Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have...

journal_title：Biotechnology letters

authors： Lee HH,Lee SJ,Kim S,Jeong S,Na M,Lee DM,Cheon YP,Lee KH,Choi I,Chun T

更新日期：2012-07-01 00:00:00

abstract：:C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...

journal_title：Biotechnology letters

authors： Kim H,Lee SY,Ji GE

更新日期：2005-09-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the ha...

journal_title：Biotechnology letters

authors： Cao Y,Peng G,He Z,Wang Z,Yin Y,Xia Y

更新日期：2007-06-01 00:00:00

abstract：:A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and t...

journal_title：Biotechnology letters

authors： Cao Y,Sun P,Fu Y,Bai X,Tian F,Liu X,Lu Z,Liu Z

更新日期：2010-09-01 00:00:00

abstract：:The genes involved in the biosynthetic pathway of ectoine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) from Bacillus halodurans were cloned as an operon and expressed in E. coli. Analysis of the deduced ectoine biosynthesis cluster amino acid sequence revealed that the ectoine operon contain 2,389 bp, enc...

journal_title：Biotechnology letters

authors： Anbu Rajan L,Joseph TC,Thampuran N,James R,Ashok Kumar K,Viswanathan C,Bansal KC

更新日期：2008-08-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...

journal_title：Biotechnology letters

authors： Foster LJ,Catzel D,Atwa S,Zarka M,Mahler SM

更新日期：2003-08-01 00:00:00

abstract：:A number of biosensors have been developed for phosphate analysis particularly, concerning its negative impact within the environmental and biological systems. Enzymatic biosensors comprising either a single or multiple enzymatic system have been extensively used for the direct and indirect analysis of phosphate ions....

journal_title：Biotechnology letters

authors： Upadhyay LS,Verma N

更新日期：2015-07-01 00:00:00

abstract：:To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...

journal_title：Biotechnology letters

authors： Yuan XF,Dai ZH,Wang XD,Zhao B

更新日期：2009-08-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 microm diam) were conjugated with the peptide, which is known...

journal_title：Biotechnology letters

authors： Kim M,Yun CH,Park SK,Seo JH,Ahn T

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflecte...

journal_title：Biotechnology letters

authors： Villanueva-Alonzo HJ,Us-Camas RY,López-Ochoa LA,Robertson D,Guerra-Peraza O,Minero-García Y,Moreno-Valenzuela OA

更新日期：2013-05-01 00:00:00

abstract：:The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 101...

journal_title：Biotechnology letters

authors： Han M,Park IS,Kim SH,Kim BS,Kim SH

更新日期：2009-11-01 00:00:00

abstract：:Baker's yeast suspensions were incubated at different pressures (from 1 bar to 6 bar) and different gases [air, O(2) and a mixture of 8% (v/v) CO(2), 21% O(2) and N(2)]. Raising the air pressure from 1 bar to 6 bar stimulated cell growth but had no effect on leavening ability or viability of the cells. A 50% reduction...

journal_title：Biotechnology letters

authors： Campelo AF,Belo I

更新日期：2004-08-01 00:00:00

abstract：:Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index ...

journal_title：Biotechnology letters

authors： Wei YH,Lai HC,Chen SY,Yeh MS,Chang JS

更新日期：2004-05-01 00:00:00

abstract：OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...

journal_title：Biotechnology letters

authors： Tardy GM,Lóránt B,Lóka M,Nagy B,László K

更新日期：2017-07-01 00:00:00