Transformation of Metarhizium anisopliae with benomyl resistance and green fluorescent protein genes provides a tag for genetically engineered strains.

abstract：:Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...

journal_title：Biotechnology letters

authors： Redwood MD,Dhillon R,Orozco RL,Zhang X,Binks DJ,Dickinson M,Macaskie LE

更新日期：2012-12-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：:Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...

journal_title：Biotechnology letters

authors： Tan H,Dan G,Gong H,Cao L

更新日期：2005-08-01 00:00:00

abstract：:Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...

journal_title：Biotechnology letters

authors： Boualem K,Gervais P,Cavin JF,Waché Y

更新日期：2014-11-01 00:00:00

abstract：OBJECTIVES:To analyze the mechanisms underlying the impact of recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)-mediated BmK IT expression on the function of baculovirus GP64 envelope fusion protein and progeny virus production. RESULTS:Viral propagation assay indicated that overexpression G...

journal_title：Biotechnology letters

authors： Fu Y,Miao Y,Zheng S,Du J,Liang A

更新日期：2016-10-01 00:00:00

abstract：OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLC...

journal_title：Biotechnology letters

authors： Wu Y,Zhang J,Hou S,Cheng Z,Yuan M

更新日期：2017-12-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...

journal_title：Biotechnology letters

authors： Nakazato G,Gonçalves MC,da Silva das Neves M,Kobayashi RKT,Brocchi M,Durán N

更新日期：2019-12-01 00:00:00

abstract：:This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...

journal_title：Biotechnology letters

authors： Hildén L,Johansson G

更新日期：2004-11-01 00:00:00

abstract：:The Bacillus pumilus SG2 chitinase gene (ChiS) and its truncated form lacking chitin binding (ChBD) and fibronectin type III (FnIII) domains were transformed to Arabidopsis plants and the expression, functionality and antifungal activity of the recombinant proteins were investigated. Results showed that while the two ...

journal_title：Biotechnology letters

authors： Dehestani A,Kazemitabar K,Ahmadian G,Jelodar NB,Salmanian AH,Seyedi M,Rahimian H,Ghasemi S

更新日期：2010-04-01 00:00:00

abstract：:Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...

journal_title：Biotechnology letters

authors： Chen JS,Wang YX,Shao L,Pan HX,Li JA,Lin HM,Dong XJ,Chen DJ

更新日期：2013-09-01 00:00:00

abstract：:Enzymatic polymerization can offer metal-free routes to polymer materials that could be used in biomedical applications. To take advantage of the unique properties of ionic liquids (ILs) for enzyme stability, monocationic ionic liquid (MIL) and dicationic ionic liquid (DIL) were used to promote the ring-opening polyme...

journal_title：Biotechnology letters

authors： Wu C,Zhang Z,He F,Zhuo R

更新日期：2013-06-01 00:00:00

abstract：:Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure ...

journal_title：Biotechnology letters

authors： Jang J,Moon SJ,Hong SH,Kim IH

更新日期：2010-11-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：OBJECTIVES:To develop a colorimetric assay for ATP based on the blue-pigment synthesising non-ribosomal peptide synthetase (NRPS) BpsA, and to demonstrate its utility in defining the substrate specificity of other NRPS enzymes. RESULTS:BpsA is able to convert two molecules of L-glutamine into the readily-detected blue...

journal_title：Biotechnology letters

authors： Brown AS,Calcott MJ,Collins VM,Owen JG,Ackerley DF

更新日期：2020-12-01 00:00:00

abstract：:We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...

journal_title：Biotechnology letters

authors： Hara KY,Suzuki R,Suzuki T,Yoshida M,Kino K

更新日期：2011-06-01 00:00:00

abstract：:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...

journal_title：Biotechnology letters

authors： Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu K

更新日期：2014-01-01 00:00:00

abstract：:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...

journal_title：Biotechnology letters

authors： Thakur D,Roy MK,Bora TC

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...

journal_title：Biotechnology letters

authors： Fouladiha H,Marashi SA,Li S,Li Z,Masson HO,Vaziri B,Lewis NE

更新日期：2021-01-01 00:00:00

abstract：:Nitrite at 2 mM did not affect the growth of Botryococcus braunii and served as the sole nitrogen source giving a minimum biomass doubling time of 4.2 d, which was equal to that of the culture using 4 mM nitrate as nitrogen source. With nitrite at 4 mM, after a lag phase of about 10 d, the alga grew quickly, reaching ...

journal_title：Biotechnology letters

authors： Yang S,Wang J,Cong W,Cai Z,Ouyang F

更新日期：2004-02-01 00:00:00

abstract：OBJECTIVES:The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro. RESULTS:By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-ti...

journal_title：Biotechnology letters

authors： Zhao Y,Wang T,Yu Z,Wang H,Liu B,Wu C,Teng CB

更新日期：2016-04-01 00:00:00

abstract：:The ability of grape skins to catalyze in vitro conversion of p-coumaric acid to the more potent antioxidant caffeic acid was studied. Addition of different concentrations of p-coumaric to red grape skins (Cabernet Sauvignon) resulted in formation of caffeic acid. This caffeic acid formation (Y) correlated positively ...

journal_title：Biotechnology letters

authors： Arnous A,Meyer AS

更新日期：2009-12-01 00:00:00

abstract：:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...

journal_title：Biotechnology letters

authors： Erhardt FA,Stammen S,Jördening HJ

更新日期：2008-06-01 00:00:00

abstract：:A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred ta...

journal_title：Biotechnology letters

authors： Reis N,Gonçalves CN,Aguedo M,Gomes N,Teixeira JA,Vicente AA

更新日期：2006-04-01 00:00:00

abstract：:Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...

journal_title：Biotechnology letters

authors： Castilho-Fernandes A,Fontes AM,Abraham KJ,de Freitas MC,da Rosa NG,Picanço-Castro V,de Sousa Russo-Carbolante EM,Covas DT

更新日期：2015-05-01 00:00:00

abstract：OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

journal_title：Biotechnology letters

authors： Wiegmann V,Martinez CB,Baganz F

更新日期：2018-07-01 00:00:00

abstract：:Nitrogenous derivatives of the two orange pigments from Monascus sp. with anti-melanogenic activities were prepared using fermentation and chemical synthesis. The pigments were produced in a 5 l jar fermentor. A total of 33 derivatives were synthesized via incorporation of L-amino acids and amines into the pigments. T...

journal_title：Biotechnology letters

authors： Jo D,Choe D,Nam K,Shin CS

更新日期：2014-08-01 00:00:00

abstract：:Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express...

journal_title：Biotechnology letters

authors： Seo HM,Jung Y,Song S,Kim Y,Kwon T,Kim DH,Jeung SJ,Yi YB,Yi G,Nam MH,Nam J

更新日期：2008-10-01 00:00:00

abstract：:Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...

journal_title：Biotechnology letters

authors： Zaynab M,Kanwal S,Furqan M,Islam W,Noman A,Ali GM,Rehman N,Zafar S,Sughra K,Jahanzab M

更新日期：2017-10-01 00:00:00

abstract：:Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...

journal_title：Biotechnology letters

authors： Foster LJ,Catzel D,Atwa S,Zarka M,Mahler SM

更新日期：2003-08-01 00:00:00