Abstract:
:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin-sensitive E. coli strain. Though expandase could not be identified, the presence of a hitherto unreported expandase in Streptomyces sp. DRS I is suggested.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Thakur D,Roy MK,Bora TCdoi
10.1007/s10529-009-9973-9subject
Has Abstractpub_date
2009-07-01 00:00:00pages
1059-64issue
7eissn
0141-5492issn
1573-6776journal_volume
31pub_type
杂志文章abstract::Different strains of Micrococcus luteus, isolated from high-altitude Argentinean wetlands, were recently reported to harbour the linear plasmids pLMA1, pLMH5 and pLMV7, all of which with 5'-covalently attached terminal proteins. The link between pLMA1 and the host's erythromycin resistance as well as further presumpti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0357-y
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2237-6
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0247-3
更新日期:2010-07-01 00:00:00
abstract::We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were success...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9532-1
更新日期:2008-02-01 00:00:00
abstract::Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6032-4
更新日期:2004-12-01 00:00:00
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journal_title:Biotechnology letters
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doi:10.1007/s10529-007-9528-x
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025072020791
更新日期:2003-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9760-z
更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024082130677
更新日期:2003-06-01 00:00:00
abstract::Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1). ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9769-3
更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025890.97067.98
更新日期:2004-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0883-x
更新日期:2012-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000003990.27624.04
更新日期:2003-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024951322955
更新日期:2003-08-01 00:00:00
abstract::A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9632-y
更新日期:2008-05-01 00:00:00
abstract::Laccase was produced by Coriolopsis rigida using barley bran as substrate in solid-state fermentation (SSF) and also by submerged fermentation (SmF). The best results were obtained in SSF with twice the amount of laccase production. Laccase could be produced from repeated batch cultures of SSF over 30 days. The laccas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9039-1
更新日期:2006-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03026-5
更新日期:2021-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9215-3
更新日期:2007-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0841-z
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2312-7
更新日期:2017-06-01 00:00:00
abstract::Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of approximately 4 x 10(6) Da with less than 0.1% protein. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9562-8
更新日期:2008-03-01 00:00:00
abstract::Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9122-7
更新日期:2006-10-01 00:00:00
abstract::Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1046-9
更新日期:2013-01-01 00:00:00
abstract::The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7879-0
更新日期:2005-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1833-1
更新日期:2015-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0824-0
更新日期:2012-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9797-z
更新日期:2008-12-01 00:00:00