Selective cleavage of 10-methyl benzo[b]naphtho[2,1-d]thiophene by recombinant Mycobacterium sp. strain.

abstract：:Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...

journal_title：Biotechnology letters

authors： Li IT,Pham E,Truong K

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...

journal_title：Biotechnology letters

authors： Etschmann MM,Sell D,Schrader J

更新日期：2003-04-01 00:00:00

abstract：:Laccase was produced by Coriolopsis rigida using barley bran as substrate in solid-state fermentation (SSF) and also by submerged fermentation (SmF). The best results were obtained in SSF with twice the amount of laccase production. Laccase could be produced from repeated batch cultures of SSF over 30 days. The laccas...

journal_title：Biotechnology letters

authors： Gómez J,Rodríguez Solar D,Pazos M,Sanromán MA

更新日期：2006-07-01 00:00:00

abstract：:The use of H(2), He and O(2) during batch fermentation of Saccharomyces cerevisiae BRAS291 increased the final intracellular glycogen contents of the cells from 2-fold to 10-fold compared with a gas-free condition, and this depended on the gas applied. Differently, the intracellular trehalose contents increased from 2...

journal_title：Biotechnology letters

authors： Pham TH,Mauvais G,Vergoignan C,De Coninck J,Cachon R,Feron G

更新日期：2008-02-01 00:00:00

abstract：:Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...

journal_title：Biotechnology letters

authors： Abbouni B,Elhariry HM,Auling G

更新日期：2003-01-01 00:00:00

abstract：:Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content ...

journal_title：Biotechnology letters

authors： Lin Y,Fang G,Peng K

更新日期：2007-02-01 00:00:00

abstract：:Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Mor...

journal_title：Biotechnology letters

authors： Tang M,Zhang RQ,Chen H,Zhang HH,Tian ZQ

更新日期：2008-10-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：:Monascus aurantiacus produces high amounts of citrinin which is a mycotoxin with nephrotoxic activity. Six putative citrinin biosynthesis genes have been discovered in M. purpureus and at least 10 genes are responsible for its biosynthesis. However, the sequence of citrinin pathway gene cluster in M. aurantiacus has n...

journal_title：Biotechnology letters

authors： Li YP,Xu Y,Huang ZB

更新日期：2012-01-01 00:00:00

abstract：OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...

journal_title：Biotechnology letters

authors： Lu F,Chen J,Ye H,Wu H,Sha F,Huang F,Cao F,Wei P

更新日期：2020-12-01 00:00:00

abstract：:Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of extracellular glyc...

journal_title：Biotechnology letters

authors： Apte-Deshpnade A,Mandal G,Soorapaneni S,Prasad B,Kumar J,Padmanabhan S

更新日期：2009-06-01 00:00:00

abstract：:Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

journal_title：Biotechnology letters

authors： Li N,Zong MH,Ma D

更新日期：2009-08-01 00:00:00

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00

abstract：:Tempe was prepared using mixtures of natural soybean and genetically modified Roundup Ready (RUR) soybean fermented with natural Rhizopus sp. The amount of RUR soybean was quantified using an ELISA plate test. The RUR signal decreased during fermentation. In the control experiments on fermentation of non-RUR soybean, ...

journal_title：Biotechnology letters

authors： Prakoso B,Nitisinprasert S,Stevens WF

更新日期：2003-09-01 00:00:00

abstract：:β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...

journal_title：Biotechnology letters

authors： Shin KC,Seo MJ,Oh DK

更新日期：2014-11-01 00:00:00

abstract：:The role of transglutaminase II (TGase II) in hyaluronic acid (HA)-promoted melanoma cell motility was investigated. HA induced the expression of TGase II via the nuclear factor kappaB (NF-kB) in melanoma cells. HA increased the Rac1 activity and phosphorylation of focal adhesion kinase (FAK). Transfection by lipofect...

journal_title：Biotechnology letters

authors： Kim Y,Park YW,Lee YS,Jeoung D

更新日期：2008-01-01 00:00:00

abstract：:An aqueous-organic biphasic system was established and used with whole cells of Oenococcus oeni to reduce 2-octanone to (R)-2-octanol. The conversion reached 99% when the Tris/borate buffer was increased from 50 mM to 300 mM in the aqueous phase. In addition, the conversion increased as the log P value of the organic ...

journal_title：Biotechnology letters

authors： Hu J,Xu Y

更新日期：2006-07-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study is to report a ι-carrageenase which degrades ι-carrageenan yielding neo-ι-carratetraose as the main product in the absence of NaCl. The gene for a new ι-carrageenase, CgiB_Ce, from Cellulophaga sp. QY3 was cloned and sequenced. It comprised an ORF of 1,386 bp encoding for a pro...

journal_title：Biotechnology letters

authors： Ma S,Tan YL,Yu WG,Han F

更新日期：2013-10-01 00:00:00

abstract：:Biodesulfurization (BDS) in a bioreactor packed with a catalytic bed of silica containing immobilized Rhodococcus rhodochrous was studied. Various bed lengths and support particle sizes were evaluated for BDS of dibenzothiophene (DBT) and gas oil. The sulfur-containing substrates were introduced separately into the bi...

journal_title：Biotechnology letters

authors： Alejandro Dinamarca M,Orellana L,Aguirre J,Baeza P,Espinoza G,Canales C,Ojeda J

更新日期：2014-08-01 00:00:00

abstract：:The volumetric productivity of the beer fermentation process can be increased by using a higher pitching rate (i.e. higher inoculum size). However, the decreased yeast net growth observed in these high cell density brewery fermentations can adversely affect the physiological stability throughout subsequent yeast gener...

journal_title：Biotechnology letters

authors： Verbelen PJ,Dekoninck TM,Van Mulders SE,Saerens SM,Delvaux F,Delvaux FR

更新日期：2009-11-01 00:00:00

abstract：:O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

journal_title：Biotechnology letters

authors： Ning H,Zhang C,Yao Y,Yu D

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVES:An extracellular protease inhibitor (BTPI-301) of trypsin was purified and characterized from an isolate of Pseudomonas mendocina. RESULTS:BTPI-301was purified to homogeneity by (NH4)2SO4, precipitation, DEAE Sepharose and CNBr-activated Sepharose chromatography. Homogeneity was proved by native PAGE and SD...

journal_title：Biotechnology letters

authors： Sapna K,Manzur Ali PP,Rekha Mol KR,Bhat SG,Chandrasekaran M,Elyas KK

更新日期：2017-12-01 00:00:00

abstract：:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...

journal_title：Biotechnology letters

authors： Erhardt FA,Stammen S,Jördening HJ

更新日期：2008-06-01 00:00:00

abstract：:The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp k...

journal_title：Biotechnology letters

authors： Zheng Z,Li H,Li L,Shao W

更新日期：2012-03-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：OBJECTIVE:The effect of epicatechin-3-gallate (ECG), a polyphenol that is present in green tea, on doxorubicin (DOX) cytotoxicity in H9C2 cardiomyocytes and its underlying mechanisms were investigated. RESULTS:Pretreatment with ECG (20 and 30 μM) significantly increased DOX-induced apoptosis to 16-18% in H9C2 cardiomy...

journal_title：Biotechnology letters

authors： Cai Y,Hong HQ,Zhao L,Cai YP,Qin Y

更新日期：2015-10-01 00:00:00

abstract：:A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan t...

journal_title：Biotechnology letters

authors： Wang G,Lu G,Ao Q,Gong Y,Zhang X

更新日期：2010-01-01 00:00:00

abstract：:The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...

journal_title：Biotechnology letters

authors： Ko BS,Rhee CH,Kim JH

更新日期：2006-08-01 00:00:00