The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes.

abstract：:KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...

journal_title：Biotechnology letters

authors： Liu Y,Ren L,Ge L,Cui Q,Cao X,Hou Y,Bai F,Bai G

更新日期：2014-08-01 00:00:00

abstract：OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...

journal_title：Biotechnology letters

authors： Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SY

更新日期：2015-11-01 00:00:00

abstract：OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...

journal_title：Biotechnology letters

authors： Stepanov A,Belyy A,Kasheverov I,Rybinets A,Dronina M,Dyachenko I,Murashev A,Knorre V,Sakharov D,Ponomarenko N,Tsetlin V,Tonevitsky A,Deyev S,Belogurov A Jr,Gabibov A

更新日期：2016-07-01 00:00:00

abstract：:Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...

journal_title：Biotechnology letters

authors： Rutter C,Chen R

更新日期：2014-02-01 00:00:00

abstract：OBJECTIVES:An extracellular protease inhibitor (BTPI-301) of trypsin was purified and characterized from an isolate of Pseudomonas mendocina. RESULTS:BTPI-301was purified to homogeneity by (NH4)2SO4, precipitation, DEAE Sepharose and CNBr-activated Sepharose chromatography. Homogeneity was proved by native PAGE and SD...

journal_title：Biotechnology letters

authors： Sapna K,Manzur Ali PP,Rekha Mol KR,Bhat SG,Chandrasekaran M,Elyas KK

更新日期：2017-12-01 00:00:00

abstract：:The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...

journal_title：Biotechnology letters

authors： Huang Y,Xie W,Yao C,Han Y,Tan G,Zhou Y,Zhu J,Pang D,Li Z,Tang X

更新日期：2014-06-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...

journal_title：Biotechnology letters

authors： Johnson EE,Jetter R,Wasteneys G

更新日期：2014-04-01 00:00:00

abstract：:The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the envi...

journal_title：Biotechnology letters

authors： Ceccato-Antonini SR

更新日期：2008-07-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：:The genus Ajuga L., belonging to Lamiaceae family, is widespread. The demand for Ajuga species has risen sharply because of their medicinal, ornamental, and pharmacological properties. These wide-ranging plants are being rapidly depleted due to over-collection for ornamental and medicinal purposes, as well as by habit...

journal_title：Biotechnology letters

authors： Park HY,Kim DH,Sivanesan I

更新日期：2017-09-01 00:00:00

abstract：OBJECTIVES:To improve the thermostability and catalytic property of a mesophilic 1,3-1,4-β-glucanase by combinational mutagenesis and to test its effect in congress mashing. RESULTS:A mutant β-glucanase (rE-BglTO) constructed by combinational mutagenesis showed a 25 °C increase in optimal temperature (to 70 °C) a 19.5...

journal_title：Biotechnology letters

authors： Niu C,Zhu L,Hill A,Alex Speers R,Li Q

更新日期：2017-01-01 00:00:00

abstract：:Over-expression of the gene, mshA, coding for mycothiol glycosyl transferase improved the robustness of Corynebacterium glutamicum to various stresses. Intracellular mycothiol (MSH) content was increased by 114 % in WT(pXMJ19-mshA) compared to WT(pXMJ19). Survival rates increased by 44, 39, 90, 77, 131, 87, 52, 47, 57...

journal_title：Biotechnology letters

authors： Liu YB,Chen C,Chaudhry MT,Si MR,Zhang L,Wang Y,Shen XH

更新日期：2014-07-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...

journal_title：Biotechnology letters

authors： Zhu BW,Huang LS,Tan HD,Qin YQ,Du YG,Yin H

更新日期：2015-02-01 00:00:00

abstract：:The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...

journal_title：Biotechnology letters

authors： Zhou S,Zhang SC,Lai DY,Zhang SL,Chen ZM

更新日期：2013-03-01 00:00:00

abstract：:The construction and testing of a unique instrument, the Paleobiosphere, which mimics some of the conditions of the ancient earth, is described. The instrument provides an experimental testing system for determining if certain microbes, when provided an adequate environment, can degrade biological materials to produce...

journal_title：Biotechnology letters

authors： Strobel G,Booth E,Schaible G,Mends MT,Sears J,Geary B

更新日期：2013-04-01 00:00:00

abstract：OBJECTIVES:A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef60-84, Nef126-144, Vpr34-47, Vpr60-75, Gp16030-53, Gp160308-323, and P248-151 epitopes was designed u...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Namazi F

更新日期：2021-01-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：:An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x...

journal_title：Biotechnology letters

authors： Aoyagi H

更新日期：2006-10-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...

journal_title：Biotechnology letters

authors： Castilho-Fernandes A,Fontes AM,Abraham KJ,de Freitas MC,da Rosa NG,Picanço-Castro V,de Sousa Russo-Carbolante EM,Covas DT

更新日期：2015-05-01 00:00:00

abstract：OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...

journal_title：Biotechnology letters

authors： Sakamoto H,Komatsu T,Yamasaki K,Satomura T,Suye SI

更新日期：2017-02-01 00:00:00

abstract：OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...

journal_title：Biotechnology letters

authors： Savvides AL,Moisi K,Katsifas EA,Karagouni AD,Hatzinikolaou DG

更新日期：2019-05-01 00:00:00

abstract：:The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the i...

journal_title：Biotechnology letters

authors： Sakai S,Liu Y,Yamaguchi T,Watanabe R,Kawabe M,Kawakami K

更新日期：2010-08-01 00:00:00

abstract：:A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...

journal_title：Biotechnology letters

authors： Kumari A,Gupta R

更新日期：2015-01-01 00:00:00

abstract：:A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and t...

journal_title：Biotechnology letters

authors： Cao Y,Sun P,Fu Y,Bai X,Tian F,Liu X,Lu Z,Liu Z

更新日期：2010-09-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：:3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...

journal_title：Biotechnology letters

authors： Yoshida T,Inami Y,Matsui T,Nagasawa T

更新日期：2010-05-01 00:00:00