Abstract:
:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneity using standard procedures. It had substrate specificity towards esters of short-chain fatty acids, particularly, p-nitrophenyl acetate. Optimum activity of EstK was at 40°C; at 4°C the activity was ~50% of its maximum. EstK has a unique substrate preference for p-nitrophenyl acetate and remains active at low temperatures.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Hong S,Lee C,Jang SHdoi
10.1007/s10529-012-0866-ysubject
Has Abstractpub_date
2012-06-01 00:00:00pages
1051-5issue
6eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
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更新日期:2004-11-01 00:00:00
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更新日期:2018-10-01 00:00:00