Abstract:
OBJECTIVES:To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. RESULTS:A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metagenomic DNA of cow dung compost. When xyn10CD18 was expressed in Bacillus megaterium MS941, extracellular xylansae activity at 106 IU/ml was achieved. The purified recombinant Xyn10CD18 was optimally active at pH 7 and 75 °C as measured over 10 min. It retained over 55% of its initial activity at 70 °C and pH 7 after 24 h. Its action on birchwood xylan for 18 h liberated xylooligosaccharides with 2°-4° of polymerization, with xylobiose and xylotetraose as the main products. When pretreated corncobs were hydrolyzed by Xyn10CD18 for 18 h, the xylooligosaccharides (DP 2-4) products increased to 80% and the xylose was just increased by 3%. CONCLUSION:Xyn10CD18 is a thermostable endoxylanase and is a promising candidate for biomass conversion and xylooligosaccharide production.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Sun MZ,Zheng HC,Meng LC,Sun JS,Song H,Bao YJ,Pei HS,Yan Z,Zhang XQ,Zhang JS,Liu YH,Lu FPdoi
10.1007/s10529-015-1857-6subject
Has Abstractpub_date
2015-09-01 00:00:00pages
1877-86issue
9eissn
0141-5492issn
1573-6776journal_volume
37pub_type
杂志文章abstract::We have cloned a novel lipase gene, lip2, from Aspergillus niger and expressed it in Escherichia coli. Upon purification of the recombinant Lip2 protein, its properties were characterized. In comparison with a previously identified lipase Lip1, both enzymes are acid lipases (optimal pH <6.5), Ca(2+)-dependent and PMSF...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0238-4
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVES:To rationally identify targets for enhancing adenosine production, transcription level of genes involved in adenosine synthesis of Bacillus subtilis XGL was detected during the fermentation process, complemented with metabolite pool analysis. RESULTS:PurR-regulated genes (pur operon and purA) and prs were d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1801-9
更新日期:2015-07-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVES:Establish a complete and efficient method for the preparation of cis-5-hydroxy-L-pipecolic acids (cis-5HPA), including biotransformation and isomers separation and purification. RESULTS:For non-heme Fe(II)/α-KG-dependent dioxygenases, α-ketoglutarate (α-KG) has great influence on the stability of Fe(II) ion...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03002-z
更新日期:2020-12-01 00:00:00
abstract::KGLP-1, a 31-amino acid glucagon-like peptide-1 (GLP-1) analogue, has a great therapeutic potential for anti-diabetes. In this work, a strategy for expression and purification of functional KGLP-1 peptide has been established. KGLP-1 cDNA was fused with glutathione S-transferase (GST), with an enterokinase cleavage si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1526-1
更新日期:2014-08-01 00:00:00
abstract::Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (I...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1165-y
更新日期:2013-06-01 00:00:00
abstract::δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1205-7
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVE:To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS:Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2368-4
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:Chiral building blocks [(S)-1-(3-methylphenyl)ethanol, (S)-1-(3,4-dimethylphenyl)ethanol and (S)-1-(2,4,6-trimethylphenyl)ethanol] for drug synthesis were prepared using two green approaches: (1) the yeast Saccharomyces cerevisiae as the biocatalyst and (2) the natural deep eutectic solvents (NADES) as the al...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2631-3
更新日期:2019-02-01 00:00:00
abstract::The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes require...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8107-2
更新日期:2005-07-01 00:00:00
abstract::A brief historical perspective on metagenomics is given followed by a discussion of the rapid progress in this field largely defined by transition to the next generation sequencing technologies. Problems and challenges connected to this transition are also addressed. The review focuses on recent literature describing ...
journal_title:Biotechnology letters
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s10529-010-0306-9
更新日期:2010-10-01 00:00:00
abstract::GERI-155 is a macrolide antibiotic containing two deoxyhexose molecules which has antimicrobial activities against gram-positive bacteria. The deoxyhexose biosynthetic gene cluster of GERI-155 from Streptomyces sp. GERI-155 genome has now been isolated. Four orf were identified and a putative orf, supposed to code for...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000013709.80691.97
更新日期:2004-02-01 00:00:00
abstract::A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9632-y
更新日期:2008-05-01 00:00:00
abstract::An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
abstract:OBJECTIVES:To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. RESULTS:The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2123-2
更新日期:2016-09-01 00:00:00
abstract::MicroRNA (miRNA) profiling is a first important step in elucidating miRNA functions. Real time quantitative PCR (RT-qPCR) and microarray hybridization approaches as well as ultra high throughput sequencing of miRNAs (small RNA-seq) are popular and widely used profiling methods. All of these profiling approaches face s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0380-z
更新日期:2010-12-01 00:00:00
abstract::The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-009-0034-1
更新日期:2009-09-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0024-3
更新日期:2009-09-01 00:00:00
abstract::A gene, rBmK Cta, encoding a chlorotoxin-like peptide from the scorpion, Buthus martensii Karsch, was synthesized according to the sequence optimized for codon usage in Escherichia coli and was expressed in E. coli BL21 (DE3) using a pExSecI expression system in which the IgG-binding domain-ZZ of protein A is fused to...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2514-2
更新日期:2005-10-01 00:00:00
abstract::16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1254-y
更新日期:2013-10-01 00:00:00
abstract::Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023068232375
更新日期:2003-04-01 00:00:00
abstract::The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9072-0
更新日期:2006-08-01 00:00:00
abstract::Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0684-6
更新日期:2005-03-01 00:00:00
abstract::Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0048-8
更新日期:2009-10-01 00:00:00
abstract::A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9942-3
更新日期:2009-06-01 00:00:00
abstract::There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical prof...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0108-0
更新日期:2010-01-01 00:00:00
abstract::In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
更新日期:2003-06-01 00:00:00
abstract:OBJECTIVE:To identify and characterize a novel bacterial pyranose 2-oxidase (P2Ox) and investigate its potential use in lignin degradation applications. RESULTS:A new bacterial P2Ox (PaP2Ox) enzyme was identified in the lignocellulolytic bacterium Pantoea ananatis Sd-1. The PaP2Ox open reading frame was cloned, and th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2538-z
更新日期:2018-05-01 00:00:00
abstract::An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutations. After precipitate...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9674-9
更新日期:2008-07-01 00:00:00