Random mutagenesis and recombination of sam1 gene by integrating error-prone PCR with staggered extension process.

abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...

journal_title：Biotechnology letters

authors： Tendulkar SR,Gupta A,Chattoo BB

更新日期：2003-11-01 00:00:00

abstract：:Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...

journal_title：Biotechnology letters

authors： Castilho-Fernandes A,Fontes AM,Abraham KJ,de Freitas MC,da Rosa NG,Picanço-Castro V,de Sousa Russo-Carbolante EM,Covas DT

更新日期：2015-05-01 00:00:00

abstract：OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Sadat SM,Irani S

更新日期：2019-11-01 00:00:00

abstract：:16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...

journal_title：Biotechnology letters

authors： Jiang D,Tu R,Bai P,Wang Q

更新日期：2013-10-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：OBJECTIVES:To prepare (R)-phenyl-1,2-ethanediol ((R)-PED) with high enantiomeric excess (ee p) and yield from racemic styrene oxide (rac-SO) at high concentration by bi-enzymatic catalysis. RESULTS:The bi-enzymatic catalysis was designed for enantioconvergent hydrolysis of rac-SO by a pair of novel epoxide hydrolases ...

journal_title：Biotechnology letters

authors： Wang R,Hu D,Zong X,Li J,Ding L,Wu M,Li J

更新日期：2017-12-01 00:00:00

abstract：:Efforts to elucidate the relationships between microorganisms and metal corrosion were mainly directed to understanding the formation of biofilm structures grown on corroded surfaces. The emergence of high throughput DNA sequencing techniques has helped in the description of microbial species involved directly and ind...

journal_title：Biotechnology letters

authors： Procópio L

更新日期：2020-03-01 00:00:00

abstract：:Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...

journal_title：Biotechnology letters

authors： Zheng Z,Jiang YH,Miao JL,Wang QF,Zhang BT,Li GY

更新日期：2006-01-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and a...

journal_title：Biotechnology letters

authors： Yomano LP,York SW,Zhou S,Shanmugam KT,Ingram LO

更新日期：2008-12-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...

journal_title：Biotechnology letters

authors： Kelly PS,Gallagher C,Clynes M,Barron N

更新日期：2015-04-01 00:00:00

abstract：:A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...

journal_title：Biotechnology letters

authors： Zhang A,Zhao R,Jin P,Ma L,Xiong X,Xie T,Pei X,Yu L,Yin X,Wang Q

更新日期：2013-11-01 00:00:00

abstract：OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...

journal_title：Biotechnology letters

authors： Sakamoto H,Komatsu T,Yamasaki K,Satomura T,Suye SI

更新日期：2017-02-01 00:00:00

abstract：:The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei. ...

journal_title：Biotechnology letters

authors： Capparelli R,Viscardi M,Amoroso MG,Blaiotta G,Bianco M

更新日期：2004-08-01 00:00:00

abstract：:The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...

journal_title：Biotechnology letters

authors： Li Z,Sun M,Li Q,Li A,Zhang C

更新日期：2012-11-01 00:00:00

abstract：:Antibody drug conjugates (ADCs) have emerged as a viable option in targeted delivery of highly potent cytotoxic drugs in treatment of solid tumors. At the time of writing, only two ADCs have received regulatory approval with >40 others in clinical development. The first generation ADCs suffered from a lack of specific...

journal_title：Biotechnology letters

authors： Bakhtiar R

更新日期：2016-10-01 00:00:00

abstract：:The rpiB gene, encoding ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum, was cloned and expressed in Escherichia coli. RpiB converted D-psicose into D-allose but it did not convert D-xylose, L-rhamnose, D-altrose or D-galactose. The production of D-allose by RpiB was maximal at pH 7.5 and 65 degrees ...

journal_title：Biotechnology letters

authors： Park CS,Yeom SJ,Kim HJ,Lee SH,Lee JK,Kim SW,Oh DK

更新日期：2007-09-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...

journal_title：Biotechnology letters

authors： van Eijsden RG,Stassen C,Daenen L,Van Mulders SE,Bapat PM,Siewers V,Goossens KV,Nielsen J,Delvaux FR,Van Hummelen P,Devreese B,Willaert RG

更新日期：2013-06-01 00:00:00

abstract：:A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

journal_title：Biotechnology letters

authors： Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

更新日期：1987-12-01 00:00:00

abstract：:Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...

journal_title：Biotechnology letters

authors： Morales M,Velázquez E,Jan J,Revah S,González U,Razo-Flores E

更新日期：2004-02-01 00:00:00

abstract：:Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...

journal_title：Biotechnology letters

authors： Sahu SK,Gopala Krishna A,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：OBJECTIVES:For the stress from fermenters, downstream processing equipment, and wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis process is of great significance in the moderately halotolerant Halomonas hydrothermalis Y2. RESULTS:In H. hydrothermalis Y2, the σ70- and σ38-control...

journal_title：Biotechnology letters

authors： Li S,Shang Y,Zhao Q,Liu Y,Dong X,Wang W,Yang C

更新日期：2021-01-27 00:00:00

abstract：:The construction and testing of a unique instrument, the Paleobiosphere, which mimics some of the conditions of the ancient earth, is described. The instrument provides an experimental testing system for determining if certain microbes, when provided an adequate environment, can degrade biological materials to produce...

journal_title：Biotechnology letters

authors： Strobel G,Booth E,Schaible G,Mends MT,Sears J,Geary B

更新日期：2013-04-01 00:00:00

abstract：:A novel cDNA for the flavonoid-3',5'-hydroxylase (F35H) gene was cloned from petals of Phalaenopsis, and designated as Phf35h (accession number DQ148458 in GenBank/EMBL/DDBJ). The genomic clone of Phf35h was isolated by a PCR-based strategy. Nucleotide sequence analysis revealed that its genomic clone contains one int...

journal_title：Biotechnology letters

authors： Wang J,Ming F,Han Y,Shen D

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVES:To prepare recombinant tPep-(vascular endothelial growth factor) VEGF-B and assess its biological activity. RESULTS:This new VEGF fusion protein was constructed using a targeting peptide and prepared using E.coli. The tPep-VEGF-B was refolded from inclusion bodies and purified using affinity chromatography....

journal_title：Biotechnology letters

authors： Zhang Y,Tong Y,Gao M,Luo C,Song X,Gao X,Yao W

更新日期：2016-07-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...

journal_title：Biotechnology letters

authors： Li Y,Liu L,Tian P

更新日期：2014-11-01 00:00:00

abstract：:Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond wi...

journal_title：Biotechnology letters

authors： Andrioli WJ,Damásio AR,Silva TM,da Silva VB,Maller A,Nanayakkara NP,Silva CH,Polizeli ML,Bastos JK

更新日期：2012-08-01 00:00:00