Characterization of ribose-5-phosphate isomerase of Clostridium thermocellum producing D-allose from D-psicose.

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...

journal_title：Biotechnology letters

authors： Fu Y,Zheng S,Huang R,An N,Zheng Y,Zhang Z,Liang A

更新日期：2012-01-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptak...

journal_title：Biotechnology letters

authors： Ma C,Yin G,You F,Wei Y,Huang Z,Chen X,Yan D

更新日期：2013-11-01 00:00:00

abstract：:Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...

journal_title：Biotechnology letters

authors： Suzuki H,Ishii J,Kondo A,Yoshida K

更新日期：2015-02-01 00:00:00

abstract：:β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...

journal_title：Biotechnology letters

authors： Shin KC,Seo MJ,Oh DK

更新日期：2014-11-01 00:00:00

abstract：:Two partial amphiploid lines, B113 (32 plants) and B21 (13 plants), derived from a wheat-Psathyrostachys huashanica intergeneric cross were characterized by Giemsa C-banding and SDS-PAGE and evaluated for stripe rust resistance. All 15 partial amphiploid plants were aneuploids with either 50 (8 plants), 51 (6 plants) ...

journal_title：Biotechnology letters

authors： Wang Y,Xie Q,Yu K,Poysa V,Lin L,Kang H,Fan X,Sha L,Zhang H,Zhou Y

更新日期：2011-06-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：:A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...

journal_title：Biotechnology letters

authors： Jin MJ,Huang H,Xiao AH,Zhang K,Liu X,Li S,Peng C

更新日期：2008-06-01 00:00:00

abstract：:A new full-length beta-1,3-glucanase cDNA, MpGlu, was isolated from a plantain (Musa paradisica) by the rapid amplification of cDNA ends (RACE) technique. Recombinant GST-MpGlu protein, expressed in E. coli, hydrolyzed (1-->3),(1-->6)-beta-glucan of Laminaria digitata and inhibited the growth of Fusarium oxysporum fsp...

journal_title：Biotechnology letters

authors： Jin X,Feng D,Wang H,Wang J

更新日期：2007-09-01 00:00:00

abstract：:Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...

journal_title：Biotechnology letters

authors： Marchev A,Haas C,Schulz S,Georgiev V,Steingroewer J,Bley T,Pavlov A

更新日期：2014-02-01 00:00:00

abstract：:C3H/HeJ mice were sensitized with ovalbumin (OVA) and choleratoxin (CT) for 5 weeks, and then Bifidobacterium bifidum BGN4 was administered continuously for 7 weeks, starting 2 weeks before (pre-treatment group) and 2 weeks after (post-treatment group) the initial sensitization. After sensitization, the OVA-induced (s...

journal_title：Biotechnology letters

authors： Kim H,Lee SY,Ji GE

更新日期：2005-09-01 00:00:00

abstract：:Ammonium is a substrate of the anaerobic ammonium oxidation (Anammox) process but it has been suggested as a substrate-inhibitor because of the action of its unionized form, free ammonia. High pH of the medium is also an important limiting factor of the Anammox bacteria. Both effects are difficult to discriminate. In ...

journal_title：Biotechnology letters

authors： Puyol D,Carvajal-Arroyo JM,Li GB,Dougless A,Fuentes-Velasco M,Sierra-Alvarez R,Field JA

更新日期：2014-10-01 00:00:00

abstract：:The role of transglutaminase II (TGase II) in hyaluronic acid (HA)-promoted melanoma cell motility was investigated. HA induced the expression of TGase II via the nuclear factor kappaB (NF-kB) in melanoma cells. HA increased the Rac1 activity and phosphorylation of focal adhesion kinase (FAK). Transfection by lipofect...

journal_title：Biotechnology letters

authors： Kim Y,Park YW,Lee YS,Jeoung D

更新日期：2008-01-01 00:00:00

abstract：:Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...

journal_title：Biotechnology letters

authors： Liang P,Qin B,Mu M,Zhang X,Jia X,You S

更新日期：2013-09-01 00:00:00

abstract：:Nitrogenous derivatives of the two orange pigments from Monascus sp. with anti-melanogenic activities were prepared using fermentation and chemical synthesis. The pigments were produced in a 5 l jar fermentor. A total of 33 derivatives were synthesized via incorporation of L-amino acids and amines into the pigments. T...

journal_title：Biotechnology letters

authors： Jo D,Choe D,Nam K,Shin CS

更新日期：2014-08-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：:The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...

journal_title：Biotechnology letters

authors： Kobayashi T

更新日期：2011-10-01 00:00:00

abstract：:The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...

journal_title：Biotechnology letters

authors： Shatila F,Yaşa İ,Yalçın HT

更新日期：2020-10-01 00:00:00

abstract：:Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...

journal_title：Biotechnology letters

authors： Zaynab M,Kanwal S,Furqan M,Islam W,Noman A,Ali GM,Rehman N,Zafar S,Sughra K,Jahanzab M

更新日期：2017-10-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:A new cDNA (Os beta fruct2) encoding an acid beta-D-fructofuranosidase from rice has been cloned, sequenced and expressed in Pichia pastoris. The full-length cDNA is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. The cDNA fragment coding for mature enzyme was sub-cloned into vector pPICZalphaA ...

journal_title：Biotechnology letters

authors： Fu RH,Wang YL,Sung HY

更新日期：2003-03-01 00:00:00

abstract：OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

journal_title：Biotechnology letters

authors： Li K,Varden L,Henderson A,Lufkin T,Kraus P

更新日期：2021-01-01 00:00:00

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVES:To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS:The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the...

journal_title：Biotechnology letters

authors： Li P,Zhou Y,Li Q,Zhang C,Sun Z,Tian L,Weng H

更新日期：2016-09-01 00:00:00