Hydrolytic potential of five fungal supernatants to enhance a commercial enzyme cocktail.

abstract：:Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...

journal_title：Biotechnology letters

authors： Zheng Z,Jiang YH,Miao JL,Wang QF,Zhang BT,Li GY

更新日期：2006-01-01 00:00:00

abstract：:Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...

journal_title：Biotechnology letters

authors： Suzuki H,Ishii J,Kondo A,Yoshida K

更新日期：2015-02-01 00:00:00

abstract：:Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...

journal_title：Biotechnology letters

authors： Marchev A,Haas C,Schulz S,Georgiev V,Steingroewer J,Bley T,Pavlov A

更新日期：2014-02-01 00:00:00

abstract：:Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of ar...

journal_title：Biotechnology letters

authors： Lee DW,Baney R

更新日期：2004-05-01 00:00:00

abstract：:A computational search was carried out to identify additional binding sites for the manganese response regulator, ManR, in the genome of Anabaena sp. PCC 7120. This approach predicted ManR binding sites: the promoter regions of the genes of all3575-alr3576 and the gene of alr5134 from Anabaena sp. PCC 7120. Electropho...

journal_title：Biotechnology letters

authors： Huang W,Wu QY

更新日期：2004-09-01 00:00:00

abstract：:A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned into prokaryotic expression vector pTrcHisA, and transformed into Escherichia coli strain Rosetta (DE3). Less t...

journal_title：Biotechnology letters

authors： Yu M,Yu M,Ru B

更新日期：2004-11-01 00:00:00

abstract：:Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...

journal_title：Biotechnology letters

authors： Wu XB,Tian XY,Ji JJ,Wu WB,Fan KQ,Yang KQ

更新日期：2011-04-01 00:00:00

abstract：:Antibody drug conjugates (ADCs) have emerged as a viable option in targeted delivery of highly potent cytotoxic drugs in treatment of solid tumors. At the time of writing, only two ADCs have received regulatory approval with >40 others in clinical development. The first generation ADCs suffered from a lack of specific...

journal_title：Biotechnology letters

authors： Bakhtiar R

更新日期：2016-10-01 00:00:00

abstract：:A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred ta...

journal_title：Biotechnology letters

authors： Reis N,Gonçalves CN,Aguedo M,Gomes N,Teixeira JA,Vicente AA

更新日期：2006-04-01 00:00:00

abstract：:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...

journal_title：Biotechnology letters

authors： Tounsi S,Jaoua S

更新日期：2003-08-01 00:00:00

abstract：:Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...

journal_title：Biotechnology letters

authors： Shi C,Shi X

更新日期：2006-02-01 00:00:00

abstract：:Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...

journal_title：Biotechnology letters

authors： Levisauskas D,Galvanauskas V,Zunda G,Grigiskis S

更新日期：2004-07-01 00:00:00

abstract：:Pectinase was immobilized on a sodium alginate support using glutaraldehyde and retained 66% activity. The optimal pH for activity shifted from 3.0 to 3.5 after immobilization; however, the optimum temperature remained unchanged at 40 degrees C. The immobilized enzyme also had a higher thermal stability and reusabilit...

journal_title：Biotechnology letters

authors： Li T,Wang N,Li S,Zhao Q,Guo M,Zhang C

更新日期：2007-09-01 00:00:00

abstract：:The rhizosphere is the soil-plant root interphase and in practice consists of the soil adhering to the root besides the loose soil surrounding it. Plant growth-promoting rhizobacteria (PGPR) are potential agents for the biological control of plant pathogens. A biocontrol strain should be able to protect the host plant...

journal_title：Biotechnology letters

authors： Babalola OO

更新日期：2010-11-01 00:00:00

abstract：:Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...

journal_title：Biotechnology letters

authors： Roessl U,Nahálka J,Nidetzky B

更新日期：2010-03-01 00:00:00

abstract：OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...

journal_title：Biotechnology letters

authors： Chung WY,Song M,Park J,Namkung W,Lee J,Kim H,Lee MG,Kim JY

更新日期：2016-12-01 00:00:00

abstract：:To develop novel antibiotic peptides useful as therapeutic drugs, a number of analogues were designed to increase the hydrophobic helix region either by Trp-substitution or net positive charge increase by Lys-substitution, from HP(2-9)-ME(1-12). The antibiotic activities of these peptides were evaluated using bacteria...

journal_title：Biotechnology letters

authors： Park Y,Kim HN,Park SN,Jang SH,Choi CH,Lim HT,Hahm KS

更新日期：2004-03-01 00:00:00

abstract：:The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have m...

journal_title：Biotechnology letters

authors： Su K,Wang C

更新日期：2015-11-01 00:00:00

abstract：:Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin alpha and L: -beta-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is we...

journal_title：Biotechnology letters

authors： Abrunhosa L,Venâncio A

更新日期：2007-12-01 00:00:00

abstract：:Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptak...

journal_title：Biotechnology letters

authors： Ma C,Yin G,You F,Wei Y,Huang Z,Chen X,Yan D

更新日期：2013-11-01 00:00:00

abstract：:The exploded bast, branch and stem of cotton stalk were degraded by alkalophilic Bacillus NT-19, with weight losses of 24%, 20% and 14%, respectively, after 14 d. Compared with a white-rot fungus (Phanerochaete chrysosporium), Bacillus NT- 19 preferentially degraded the non-cellulose components of cotton stem. The rel...

journal_title：Biotechnology letters

authors： Zheng L,Han X,Du Y

更新日期：2003-10-01 00:00:00

abstract：:UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation o...

journal_title：Biotechnology letters

authors： Liu W,Lu X,He G,Gao X,Li M,Wu J,Li Z,Wu J,Wang J,Luo C

更新日期：2013-04-01 00:00:00

abstract：:1-Propyl laurate synthesis should not be used as standard reaction test of immobilized enzymes in microemulsion-based organogels (MBGs) prepared using lecithin/1-propanol as surfactant when extremely active enzymes with high load are used. In these cases, an anomalous kinetic reaction constant value is observed over s...

journal_title：Biotechnology letters

authors： Domínguez de María P,Xenakis A,Stamatis H,Sinisterra JV

更新日期：2004-10-01 00:00:00

abstract：OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...

journal_title：Biotechnology letters

authors： Techaparin A,Thanonkeo P,Klanrit P

更新日期：2017-10-01 00:00:00

abstract：:A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan t...

journal_title：Biotechnology letters

authors： Wang G,Lu G,Ao Q,Gong Y,Zhang X

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...

journal_title：Biotechnology letters

authors： Onraedt A,De Mey M,Walcarius B,Soetaert W,Vandamme EJ

更新日期：2006-11-01 00:00:00

abstract：:Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...

journal_title：Biotechnology letters

authors： Mukundan LM,Nirmal R,Thomas LV,Sajeev US,Nair PD

更新日期：2011-10-01 00:00:00

abstract：:3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...

journal_title：Biotechnology letters

authors： Yoshida T,Inami Y,Matsui T,Nagasawa T

更新日期：2010-05-01 00:00:00

abstract：:Bacterial and fungal contaminants of enset (Ensete ventricosum) cultures and microbes associated with surface-sterilized field material were identified by 16S/26S rDNA sequencing. Ten bacterial species were identified in 16 isolates from in vitro cultures and seven in 10 isolates from field clones. Three yeast species...

journal_title：Biotechnology letters

authors： Birmeta G,Passoth V,Roos S,Welander M

更新日期：2004-12-01 00:00:00