Abstract:
:Pectinase was immobilized on a sodium alginate support using glutaraldehyde and retained 66% activity. The optimal pH for activity shifted from 3.0 to 3.5 after immobilization; however, the optimum temperature remained unchanged at 40 degrees C. The immobilized enzyme also had a higher thermal stability and reusability than the free enzyme, and retained 80% of initial activity after 11 batch reactions.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Li T,Wang N,Li S,Zhao Q,Guo M,Zhang Cdoi
10.1007/s10529-007-9409-3subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1413-6issue
9eissn
0141-5492issn
1573-6776journal_volume
29pub_type
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journal_title:Biotechnology letters
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doi:10.1007/s10529-008-9760-z
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abstract::β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1604-4
更新日期:2014-11-01 00:00:00
abstract::Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...
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pub_type: 杂志文章
doi:10.1007/s10529-012-1032-2
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1019-z
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...
journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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doi:10.1007/s10529-010-0442-2
更新日期:2011-02-01 00:00:00
abstract::We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1400-6
更新日期:2014-03-01 00:00:00
abstract::An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0866-y
更新日期:2012-06-01 00:00:00
abstract::Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8457-9
更新日期:2005-08-01 00:00:00
abstract::The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0982-8
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2398-y
更新日期:2017-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2314-5
更新日期:2017-06-01 00:00:00
abstract::The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at positi...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9020-z
更新日期:2006-06-01 00:00:00
abstract::Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-005-1521-7
更新日期:2005-03-01 00:00:00
abstract:OBJECTIVE:To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS:Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2368-4
更新日期:2017-09-01 00:00:00
abstract::Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03010-z
更新日期:2020-12-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2099-y
更新日期:2016-07-01 00:00:00
abstract::The biomacromolecule, gelatin, has increasingly been used in biomedicine-beyond its traditional use in food and cosmetics. The appealing advantages of gelatin, such as its cell-adhesive structure, low cost, off-the-shelf availability, high biocompatibility, biodegradability and low immunogenicity, among others, have m...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1907-0
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2101-8
更新日期:2016-08-01 00:00:00
abstract::Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9621-1
更新日期:2008-05-01 00:00:00
abstract::5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0943-2
更新日期:2012-09-01 00:00:00
abstract::Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-013-1350-z
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2338-x
更新日期:2017-07-01 00:00:00
abstract::Some recombinant proteins expressed by baculovirus expression vector systems (BEVS) aggregate because the BEVS can produce large amounts of protein late during infection, when post-translational modification and protein quality control mechanisms are inactive. For expression during earlier stages than that driven by t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0340-7
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVES:L-isoleucine dioxygenase (IDO) specifically transforms L-isoleucine (Ile) to 4-hydroxyisoleucine (4-HIL), and 4-HIL is a promising drug for diabetes. To enhance the activity and catalytic efficiency of IDO, we used directed evolution and site-specific mutagenesis. RESULTS:The IDO gene (ido) derived from Bac...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2566-8
更新日期:2018-08-01 00:00:00