Abstract:
:A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium decreased the initial rate in all cases tested. The operational stability during Z-Gly-Phe-NH2 synthesis was improved when solid cysteine was added, doubling the yield after 24 h.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Theppakorn T,Kanasawud P,Halling PJdoi
10.1023/b:bile.0000012891.13904.62keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
133-6issue
2eissn
0141-5492issn
1573-6776journal_volume
26pub_type
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