Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans.

Abstract:

:Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.

journal_name

Biotechnol Lett

journal_title

Biotechnology letters

authors

Senthilkumar M,Madhaiyan M,Sundaram SP,Sangeetha H,Kannaiyan S

doi

10.1007/s10529-008-9693-6

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

1477-87

issue

8

eissn

0141-5492

issn

1573-6776

journal_volume

30

pub_type

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