Cardiac cells implanted into a cylindrical, vascularized chamber in vivo: pressure generation and morphology.

abstract：:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...

journal_title：Biotechnology letters

authors： Thakur D,Roy MK,Bora TC

更新日期：2009-07-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...

journal_title：Biotechnology letters

authors： Li Z,Sun M,Li Q,Li A,Zhang C

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：:A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...

journal_title：Biotechnology letters

authors： Theppakorn T,Kanasawud P,Halling PJ

更新日期：2004-01-01 00:00:00

abstract：:Heterologous expression in Escherichia coli often leads to production of the expressed proteins as insoluble and inactive inclusion bodies. The general strategy for protein recovery includes isolation and washing of inclusion bodies, solubilization of aggregated protein and refolding of solubilized protein. The proces...

journal_title：Biotechnology letters

authors： Ferreira S,Sousa F,Queiroz JA,Domingues FC

更新日期：2005-08-01 00:00:00

abstract：:Nitrite at 2 mM did not affect the growth of Botryococcus braunii and served as the sole nitrogen source giving a minimum biomass doubling time of 4.2 d, which was equal to that of the culture using 4 mM nitrate as nitrogen source. With nitrite at 4 mM, after a lag phase of about 10 d, the alga grew quickly, reaching ...

journal_title：Biotechnology letters

authors： Yang S,Wang J,Cong W,Cai Z,Ouyang F

更新日期：2004-02-01 00:00:00

abstract：:A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...

journal_title：Biotechnology letters

authors： Jin MJ,Huang H,Xiao AH,Zhang K,Liu X,Li S,Peng C

更新日期：2008-06-01 00:00:00

abstract：:The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...

journal_title：Biotechnology letters

authors： Bisen PS,Sanodiya BS,Thakur GS,Baghel RK,Prasad GB

更新日期：2010-08-01 00:00:00

abstract：OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...

journal_title：Biotechnology letters

authors： Zhang Y,Yang L,Ling C,Heng W

更新日期：2018-04-01 00:00:00

abstract：:A Simplified version of rate equations for enzyme-catalyzed reactions has been developed in which the rate equation is analyzed with two different mechanisms: ordered bi-ter and Ping-Pong bi-bi mechanisms. This procedure is able to develop the rate expressions accurately. Random sequential order mechanism can be effec...

journal_title：Biotechnology letters

authors： Thilakavathi M,Basak T,Panda T

更新日期：2006-12-01 00:00:00

abstract：:An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...

journal_title：Biotechnology letters

authors： Hwang SH,Koo YM

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:To uncover key genes and pathways regulated by SCL3, a GRAS transcription factor, in the context of gibberellin (GA) in the roots of the model plant Arabidopsis thaliana. RESULTS:Gene expression profiles of ga1-3 mutant and ga1-3 and scl3 double mutant are considerably similar to each other, revealed by Pri...

journal_title：Biotechnology letters

authors： Weng CY,Zhu MH,Liu ZQ,Zheng YG

更新日期：2020-06-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：OBJECTIVES:The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro. RESULTS:By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-ti...

journal_title：Biotechnology letters

authors： Zhao Y,Wang T,Yu Z,Wang H,Liu B,Wu C,Teng CB

更新日期：2016-04-01 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：:Two partial amphiploid lines, B113 (32 plants) and B21 (13 plants), derived from a wheat-Psathyrostachys huashanica intergeneric cross were characterized by Giemsa C-banding and SDS-PAGE and evaluated for stripe rust resistance. All 15 partial amphiploid plants were aneuploids with either 50 (8 plants), 51 (6 plants) ...

journal_title：Biotechnology letters

authors： Wang Y,Xie Q,Yu K,Poysa V,Lin L,Kang H,Fan X,Sha L,Zhang H,Zhou Y

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVE:To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production. RESULTS:Monoamine oxidase, originating from Aspergillus niger and subj...

journal_title：Biotechnology letters

authors： Markošová K,Camattari A,Rosenberg M,Glieder A,Turner NJ,Rebroš M

更新日期：2018-01-01 00:00:00

abstract：:Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...

journal_title：Biotechnology letters

authors： Etschmann MM,Sell D,Schrader J

更新日期：2003-04-01 00:00:00

abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...

journal_title：Biotechnology letters

authors： Turner KM,Pasut G,Veronese FM,Boyce A,Walsh G

更新日期：2011-03-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...

journal_title：Biotechnology letters

authors： Wang W,Sun J,Xiao W,Jiang L,Wang R,Fan J

更新日期：2017-11-01 00:00:00

abstract：OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...

journal_title：Biotechnology letters

authors： Zhang B,Song Y,Chen H,Zhang H,Chen W

更新日期：2016-11-01 00:00:00

abstract：:Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...

journal_title：Biotechnology letters

authors： Patett F,Fischer L

更新日期：2005-12-01 00:00:00

abstract：PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inh...

journal_title：Biotechnology letters

authors： Niu C,Cai M,Zhang Y,Zhou X

更新日期：2012-11-01 00:00:00

abstract：:Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...

journal_title：Biotechnology letters

authors： Roessl U,Nahálka J,Nidetzky B

更新日期：2010-03-01 00:00:00

abstract：:Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...

journal_title：Biotechnology letters

authors： Kuan IC,Chuang CA,Lee SL,Yu CY

更新日期：2012-08-01 00:00:00

abstract：:A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...

journal_title：Biotechnology letters

authors： Jeromin GE,Zoor A

更新日期：2008-05-01 00:00:00

abstract：:Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...

journal_title：Biotechnology letters

authors： Berne C,Allainmat B,Garcia D

更新日期：2005-04-01 00:00:00