Abstract:
OBJECTIVES:To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate. RESULTS:Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1-4. Antioxidative peptides were separated by sequential acid precipitation, cation exchange, and reversed-phase fast performance liquid chromatography. Finally, a peptide with antioxidative activity was identified as Ser-Asn-Leu-Cys-Arg-Pro-Cys-Gly by MALDI time-of-flight (TOF)/TOF analysis, and determined to represent a portion of feather keratin near its N-terminal. A synthesized peptide with the same sequence was used to characterize its antioxidative properties, including scavenging free radicals, reducing power, and Fe(2+) chelation. In terms of the peptide's amino acid composition, the antioxidative activity might be mainly attributed to Cys and other amino acid residues. CONCLUSION:Feather keratin is a good source for the quantitative preparation of antioxidative peptides.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Wan MY,Dong G,Yang BQ,Feng Hdoi
10.1007/s10529-015-2016-9subject
Has Abstractpub_date
2016-04-01 00:00:00pages
643-9issue
4eissn
0141-5492issn
1573-6776pii
10.1007/s10529-015-2016-9journal_volume
38pub_type
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