Abstract:
:A gene cassette, p35S-CNO, was designed to express three gene products driven by a single constitutive CaMV 35S promoter. The individual coding regions were linked in frame to produce a single polyprotein, using spacer sequences encoding a specific heptapeptide cleavage recognition site (ENLYFQS) for the nuclear-inclusion-a (NIa) proteinase of tobacco etch virus (TEV). The protein coding sequences used were: a Trichoderma harzinum endochitinase, a truncated NIa proteinase of TEV, and a wheat oxalate oxidase. When p35S-CNO construct was tested in Arabidopsis thaliana, the polyprotein was properly cleaved after translation and the products exhibited functional enzymatic activity in vivo.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Liang H,Gao H,Maynard CA,Powell WAdoi
10.1007/s10529-005-1884-9keywords:
subject
Has Abstractpub_date
2005-03-01 00:00:00pages
435-42issue
6eissn
0141-5492issn
1573-6776journal_volume
27pub_type
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