Production of a recombinant chitin oligosaccharide deacetylase from Vibrio parahaemolyticus in the culture medium of Escherichia coli cells.

abstract：:Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...

journal_title：Biotechnology letters

authors： Trung TS,Ng CH,Stevens WF

更新日期：2003-07-01 00:00:00

abstract：:Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also a...

journal_title：Biotechnology letters

authors： Gomes N,Teixeira JA,Belo I

更新日期：2012-04-01 00:00:00

abstract：:Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...

journal_title：Biotechnology letters

authors： Shu X,Ma Y,Lu C,Lei M,Liu Y,Liu K,Xiong G,Xia S,Zhao Z,Luo W,Fu Q,Qi C

更新日期：2015-05-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：:A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of Sig...

journal_title：Biotechnology letters

authors： Lee BR,Kim JH

更新日期：2004-04-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：:Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...

journal_title：Biotechnology letters

authors： Tan H,Dan G,Gong H,Cao L

更新日期：2005-08-01 00:00:00

abstract：:Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-05-01 00:00:00

abstract：OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Sadat SM,Irani S

更新日期：2019-11-01 00:00:00

abstract：:A new cDNA (Os beta fruct2) encoding an acid beta-D-fructofuranosidase from rice has been cloned, sequenced and expressed in Pichia pastoris. The full-length cDNA is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. The cDNA fragment coding for mature enzyme was sub-cloned into vector pPICZalphaA ...

journal_title：Biotechnology letters

authors： Fu RH,Wang YL,Sung HY

更新日期：2003-03-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...

journal_title：Biotechnology letters

authors： Zhang B,Song Y,Chen H,Zhang H,Chen W

更新日期：2016-11-01 00:00:00

abstract：:A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...

journal_title：Biotechnology letters

authors： Park CS,Kim JE,Lee SH,Kim YS,Kang LW,Oh DK

更新日期：2013-11-01 00:00:00

abstract：:Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful...

journal_title：Biotechnology letters

authors： Masroori N,Halabian R,Mohammadipour M,Roushandeh AM,Rouhbakhsh M,Najafabadi AJ,Fathabad ME,Salimi M,Shokrgozar MA,Roudkenar MH

更新日期：2010-06-01 00:00:00

abstract：:In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...

journal_title：Biotechnology letters

authors： Shi Y,Zhao XT,Cao P,Hu Y,Zhang L,Jia Y,Lu Z

更新日期：2009-09-01 00:00:00

abstract：:A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...

journal_title：Biotechnology letters

authors： Zhang A,Zhao R,Jin P,Ma L,Xiong X,Xie T,Pei X,Yu L,Yin X,Wang Q

更新日期：2013-11-01 00:00:00

abstract：:The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various...

journal_title：Biotechnology letters

authors： Park JH,Song MH,Lee CH,Lee MK,Park YM,Old L,Lee SY

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVES:To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate. RESULTS:Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1-4. Antioxidative peptides were separated by sequential acid pr...

journal_title：Biotechnology letters

authors： Wan MY,Dong G,Yang BQ,Feng H

更新日期：2016-04-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：:Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...

journal_title：Biotechnology letters

authors： Park Y,Lee S,Kang H,Park M,Min K,Kim NH,Gu S,Kim JK,An DJ,Choe S,Sohn EJ

更新日期：2020-07-01 00:00:00

abstract：OBJECTIVES:The objective of this study was to discover unknown differentially expressed genes (DEGs) associated with bronchopulmonary dysplasia (BPD), analyze their functions and enriched signaling pathways, and identify hub genes correlating with BPD incidence and evolvement. RESULTS:Of 1289 DEGs identified, 568 were...

journal_title：Biotechnology letters

authors： Yan W,Jiang M,Zheng J

更新日期：2020-12-01 00:00:00

abstract：OBJECTIVES:To improve target protein production by manipulating expression levels of alanine racemase in Bacillus licheniformis. RESULTS:The gene of dal was identified to be responsible for alanine racemase function. Based on the selection marker of dal, a food-grade expression system was constructed in B. licheniform...

journal_title：Biotechnology letters

authors： He P,Zhang Z,Cai D,Chen Y,Wang H,Wei X,Li S,Chen S

更新日期：2017-09-01 00:00:00

abstract：:A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...

journal_title：Biotechnology letters

authors： Jeromin GE,Zoor A

更新日期：2008-05-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...

journal_title：Biotechnology letters

authors： Theppakorn T,Kanasawud P,Halling PJ

更新日期：2004-01-01 00:00:00

abstract：:A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two...

journal_title：Biotechnology letters

authors： Ming-Ming Y,Wei-Wei Z,Xi-Feng Z,Pei-Lin C

更新日期：2006-11-01 00:00:00

abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily by enzymic modification of these drugs. One important mechanism of streptomycin modification is through ATP-dependent O-adenylation, catalyzed by streptomycin adenylyltransferase. Initial velocity patterns deduced from steady state kinetics...

journal_title：Biotechnology letters

authors： Jana S,Deb JK

更新日期：2005-04-01 00:00:00

abstract：:A fusion protein consisting of streptavidin and firefly luciferase was constructed to establish an accurate measuring technique of local ATP concentration. The fusion protein retained the binding ability of streptavidin and enzymatic activity of luciferase. Also, it could detect the concentration of antigens and could...

journal_title：Biotechnology letters

authors： Nakamura M,Mie M,Funabashi H,Kobatake E

更新日期：2004-07-01 00:00:00

abstract：:The open reading frame of the Δ(6)-desaturase gene was isolated from Mortierella alpina W15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed into Pichia pastoris GS115 and expression was induced with methanol. The Δ(6)-desaturase expressed in P. pastoris GS115 catalyzed the conversion of lino...

journal_title：Biotechnology letters

authors： Liu J,Li D,Yin Y,Wang H,Li M,Yu L

更新日期：2011-10-01 00:00:00